Description

3,7-Dihydro-6-(4-(2-(N'-(5-Fluoresceinyl)Thioureido)Ethoxy)Phenyl)-2-Methylimidazo-(1,2-A)Pyrazin-3-One is a high-purity, synthetic chemical probe designed for advanced research and diagnostic applications. Its core value lies in its unique molecular architecture, which combines a fluorescein moiety with a chemiluminescent imidazopyrazinone core, enabling highly sensitive detection and quantification. This compound is essential for researchers and developers in the fields of biochemical assay development, clinical diagnostics, and life science research, where precise and reliable detection reagents are critical.

Application

• Chemiluminescent Substrate: Primary application as a highly sensitive substrate for the detection and quantification of peroxidase enzymes (e.g., Horseradish Peroxidase, HRP) in immunoassays (ELISA, Western Blotting).
• Bioluminescence Research: Used as a synthetic analog or probe in studies related to coelenterazine and other marine imidazopyrazinone-based bioluminescent systems.
• Diagnostic Kit Manufacturing: A key raw material for the formulation of high-sensitivity diagnostic kits for clinical and veterinary testing.
• Biomarker Detection: Enables the development of ultra-sensitive assays for disease biomarkers, including cardiac markers, hormones, and infectious agents.
• Cellular Imaging Probes: Potential use in the development of probes for cellular imaging and in vivo studies, leveraging its chemiluminescent and fluorescent properties.
• Research & Development: Serves as a critical reagent in academic and industrial R&D for novel assay development and signal amplification strategies.

Basic Information

Item	Details
Product Name	3,7-Dihydro-6-(4-(2-(N'-(5-Fluoresceinyl)Thioureido)Ethoxy)Phenyl)-2-Methylimidazo-(1,2-A)Pyrazin-3-One
CAS No.	132059-43-9
Molecular Formula	C35H23N5O6S
Molecular Weight	641.66 g/mol
Synonyms	6-(4-(2-(N'-(5-Fluoresceinyl)Thioureido)Ethoxy)Phenyl)-2-Methylimidazo(1,2-a)Pyrazin-3(7H)-One; Lumi-Phos 530; CSPD® (Chemiluminescent Substrate for Peroxidase Detection); 2-Methyl-6-(4-(2-(5-(N-(5-Fluoresceinyl)Thioureido)Ethoxy)Phenyl)Imidazo[1,2-a]Pyrazin-3(7H)-One; Enhanced Chemiluminescence Substrate; Peroxidase Chemiluminescent Substrate; Fluorescein-Thiourea-Imidazopyrazinone Conjugate
EINECS	Contact for details

Quality Control

Our production of this advanced chemiluminescent substrate adheres to stringent quality protocols to ensure batch-to-batch consistency and optimal performance in sensitive assays. Every lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance is provided with each shipment to support your quality management system.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a desiccated environment. Under these conditions, the product is typically stable for 24 months from the date of receipt. Allow the vial to equilibrate to room temperature in a dry, dark place before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0% (Area Percent)
Water Content (KF)	≤ 1.0 %
Residual Solvents (GC)	Meets ICH guidelines
Functional Activity	Meets internal performance standard for chemiluminescent signal generation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.