Description

Mag-Fura-2, Am is a cell-permeant, acetoxymethyl (AM) ester derivative of the fluorescent magnesium indicator Mag-Fura-2. This compound is critical for researchers requiring precise, real-time measurement of intracellular magnesium ion (Mg²⁺) concentrations. It is primarily utilized in life science research, pharmacology, and cellular physiology for studying magnesium dynamics in live cells, neuronal signaling, and mitochondrial function.

Application

• Intracellular Magnesium Imaging: As a rationetric fluorescent probe for quantifying free Mg²⁺ levels in live cells using fluorescence microscopy (e.g., Fura-2 ratio imaging techniques).
• Neuroscience Research: Investigation of the role of magnesium ions in synaptic transmission, neurotransmitter release, and neuronal excitability.
• Cardiovascular Studies: Monitoring Mg²⁺ fluctuations in cardiac myocytes to understand heart muscle contraction and related pathologies.
• Mitochondrial Function Analysis: Probing magnesium homeostasis within mitochondria to study energy metabolism and apoptosis.
• Pharmacological Screening: Used in assays to evaluate the effects of drugs or compounds on cellular magnesium regulation and transport.
• Cell Physiology & Signal Transduction: Fundamental research into magnesium's role as a secondary messenger and cofactor in enzymatic processes.

Basic Information

Product Name	Mag-Fura-2, Am
CAS No.	130100-20-8
Molecular Formula	C₃₉H₄₀N₃O₁₈
Molecular Weight	838.75 g/mol
Synonyms	Mag-fura-2 acetoxymethyl ester; Mag-Fura-2 AM; Mag-Fura-2, acetoxymethyl ester, tetraammonium salt; Magnesium indicator Mag-Fura-2 AM; Fura-2 based Mg²⁺ indicator AM ester; (2-(5-Carboxy-4-[(2-methyl-8-(trifluoromethyl)-4-quinolyl)amino]-2-furanyl)-5-oxazolyl)phosphonic acid, acetoxymethyl ester, tetraammonium salt
EINECS	Contact for details

Quality Control

Our Mag-Fura-2, Am is produced under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, NMR for structural confirmation, and functional validation in cell-based assays. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting identity, purity, and performance data.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. The product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity	Effective for intracellular Mg²⁺ imaging
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.