Description

Rhod 2 Triammonium CAS NO 129787-65-1 is a high-purity, cell-permeant calcium indicator salt widely used in fluorescence microscopy and life science research. This compound is critical for the non-invasive, real-time measurement of intracellular calcium ion (Ca²⁺) concentrations, a key parameter in cellular signaling studies. It is an essential tool for researchers and professionals in neurobiology, cardiology, pharmacology, and general cell biology who require reliable and sensitive fluorescent probes for quantitative imaging and flow cytometry.

Application

• Intracellular Calcium Imaging: Primary application for real-time visualization and quantification of cytosolic Ca²⁺ flux in live cells using fluorescence microscopy (e.g., confocal, epifluorescence).
• Neuroscience Research: Monitoring neuronal activity and synaptic transmission by detecting calcium transients associated with action potentials and neurotransmitter release.
• Cardiovascular Research: Studying calcium signaling in cardiomyocytes to understand muscle contraction, arrhythmias, and drug effects on heart cells.
• Pharmacology & Drug Discovery: High-throughput screening (HTS) assays to evaluate the effect of novel compounds on cellular calcium homeostasis and GPCR activity.
• Cell Physiology Studies: Investigating calcium's role as a second messenger in processes like cell proliferation, apoptosis, secretion, and immune response.
• Flow Cytometry: Used in conjunction with flow cytometers to analyze calcium levels in cell populations for immunology and cancer research.

Basic Information

Product Name	Rhod 2 Triammonium
CAS No.	129787-65-1
Molecular Formula	C₄₆H₆₁N₇O₁₃ · 3NH₃
Molecular Weight	~1010 g/mol (as triammonium salt)
Synonyms	Rhod-2 triammonium salt; Rhod2; Rhod 2, AM ester precursor; X-Rhod-1 analogue; Calcium indicator Rhod-2; 5-(2-(4-(Bis(carboxymethyl)amino)-3-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenyl)ethylcarbamoyl)-2-(6-(bis(carboxymethyl)amino)-5-(2-(2,5-dioxopyrrolidin-1-yl)oxy)-2-oxoethoxy)phenoxy)benzoic acid triammonium salt
EINECS	Contact for details

Quality Control

Our Rhod 2 Triammonium is manufactured under strict quality systems to ensure batch-to-batch consistency and performance reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for correct absorbance/emission profiles, and functional validation in cell-based assays. A detailed Certificate of Analysis (COA) documenting identity, purity, and performance specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption Max (λmax)	~552 nm
Emission Max (λem)	~581 nm (Ca²⁺ bound)
Extinction Coefficient (ε)	Contact for details
Calcium Binding Affinity (Kd)	~570 nM (typical)
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.