Description

Hoechst 33258 Analog 6 is a high-purity, synthetic derivative of the classic DNA-binding fluorophore Hoechst 33258. This compound is valued for its specific and enhanced binding affinity to AT-rich regions of double-stranded DNA, making it a critical tool for advanced molecular biology and cytogenetics research. It is primarily utilized by researchers and manufacturers in the biotechnology, pharmaceutical development, and diagnostic reagent sectors for applications requiring precise nucleic acid detection and quantification.

Application

• Fluorescent DNA Staining in gel electrophoresis for visualization and quantification of nucleic acids.
• Cell Cycle Analysis and ploidy determination via flow cytometry.
• Chromosome Banding and cytogenetic studies in karyotyping.
• Development of Diagnostic Kits for genetic testing and pathogen detection.
• High-Content Screening (HCS) assays in drug discovery to study nuclear morphology and DNA content.
• Research Tool for studying DNA-protein interactions and chromatin structure.
• Quality Control Reagent in the production of nucleic acid-based therapeutics and vaccines.

Basic Information

Product Name	Hoechst 33258 Analog 6
CAS No.	129244-66-2
Molecular Formula	C25H24N6O
Molecular Weight	424.50 g/mol
Synonyms	Pibenzimol analog; Bisbenzimide H 33258 analog 6; 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole; Hoechst 33258 derivative; DNA stain 33258 analog; Fluorescent DNA probe analog; Trihydrochloride salt of Hoechst 33258 analog (if applicable)
EINECS	Contact for details

Quality Control

Our Hoechst 33258 Analog 6 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometric analysis for identity and performance verification, and stringent controls for residual solvents and impurities. A Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Fluorescence Emission	Conforms to reference (λem ~465 nm in DNA complex)
Solubility	Soluble in DMSO, slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.