Description

4-Methylumbelliferyl 2,3,4-Tri-o-Acetyl-α-L-Idopyranosiduronic Acid, Methyl Ester is a specialized fluorogenic substrate designed for enzymatic research and diagnostic applications. This compound is critical for the sensitive and specific detection of glycosidase enzyme activity, particularly α-L-iduronidase, due to its tailored chemical structure. It is an essential tool for researchers and manufacturers in the fields of biochemical research, diagnostic kit development, and pharmaceutical quality control, where precise enzyme activity measurement is paramount.

Application

• Enzymatic Assay Substrate: Primary use as a fluorogenic substrate for the detection and quantification of α-L-iduronidase activity in research and clinical diagnostics.
• Lysosomal Storage Disorder Research: Critical tool for studying enzyme deficiencies related to disorders such as Mucopolysaccharidosis I (Hurler, Scheie syndromes).
• Diagnostic Kit Manufacturing: A key raw material in the production of in-vitro diagnostic (IVD) kits for newborn screening and enzyme activity tests.
• High-Throughput Screening (HTS): Used in pharmaceutical R&D for screening potential therapeutic compounds that modulate glycosidase activity.
• Biochemical Research Reagent: Employed in academic and industrial laboratories for fundamental studies on carbohydrate metabolism and enzyme kinetics.
• Quality Control in Biologics: Used to monitor enzyme activity and purity in the production of recombinant therapeutic enzymes.

Basic Information

Item	Detail
Product Name	4-Methylumbelliferyl 2,3,4-Tri-o-Acetyl-α-L-Idopyranosiduronic Acid, Methyl Ester
CAS No.	128095-50-1
Molecular Formula	C23H24O13
Molecular Weight	508.43 g/mol
Synonyms	4-MU-IdoA-OMe triacetate; 4-Methylumbelliferyl α-L-idopyranosiduronic acid methyl ester triacetate; α-L-Iduronidase substrate; Fluorogenic iduronide substrate; 4-MU-α-L-IdoA-OMe (2,3,4-tri-O-acetyl); 4-Methylumbelliferyl 2,3,4-tri-O-acetyl-α-L-idopyranosiduronic acid methyl ester; Iduronidase fluorogenic substrate
EINECS	Contact for details

Quality Control

Every batch of our 4-Methylumbelliferyl 2,3,4-Tri-o-Acetyl-α-L-Idopyranosiduronic Acid, Methyl Ester is produced and tested under a strict quality management system to ensure high purity, batch-to-batch consistency, and reliable performance in sensitive enzymatic assays. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and impurity profiles. Our quality standards are designed to meet the rigorous requirements of research-grade and diagnostic-grade chemical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the container to reach room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Fluorogenic Activity	Functional in standard α-L-iduronidase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.