Description

6-Carboxytetramethylrhodamine n-Hydroxysuccinimide Ester is a high-purity, amine-reactive fluorescent labeling reagent widely used for the covalent conjugation of biomolecules. Its core value lies in providing a stable, bright, and photostable fluorescent tag, enabling sensitive detection and analysis in complex biological systems. This product is essential for researchers and manufacturers in the fields of bioconjugation, flow cytometry, fluorescence microscopy, and in vitro diagnostics who require reliable and consistent performance for their assays and kits.

Application

• Antibody and Protein Labeling: Conjugation to primary or secondary antibodies for immunofluorescence (IF), immunohistochemistry (IHC), and Western blotting.
• Nucleic Acid Probe Synthesis: Labeling of oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Flow Cytometry Reagents: Preparation of fluorescently-tagged detection reagents for cell surface and intracellular marker analysis.
• Biomolecule Tracking: Labeling of peptides, proteins, or other biomolecules to study their localization, interaction, and turnover in live or fixed cells.
• Diagnostic Kit Manufacturing: A key component in the production of lateral flow assays, ELISA kits, and other diagnostic platforms requiring a fluorescent readout.
• Materials Science: Functionalization of nanoparticles, polymers, or surfaces with fluorescent markers for imaging and sensing applications.

Basic Information

Product Name	6-Carboxytetramethylrhodamine n-Hydroxysuccinimide Ester
CAS No.	127290-93-1
Molecular Formula	C32H31N3O7
Molecular Weight	569.61 g/mol
Synonyms	6-TAMRA NHS Ester; 6-Carboxy-tetramethylrhodamine N-succinimidyl ester; 6-TAMRA, SE; 5(6)-TAMRA NHS Ester; Tetramethylrhodamine-6-isothiocyanate isomer; 6-Carboxytetramethylrhodamine succinimidyl ester; Rhodamine 6G NHS ester (isomer); X-Rhodamine NHS Ester (common trade name)
EINECS	Contact for details

Quality Control

Our 6-Carboxytetramethylrhodamine NHS Ester is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and labeling efficiency. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence profiles, and functional performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	550 ± 5 nm
Emission Maximum (λem)	575 ± 5 nm
Extinction Coefficient (ε)	~90,000 cm-1M-1
Water Content (KF)	≤ 1.0%
Functional Performance	≥ 90% Labeling Efficiency (tested with standard amine)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.