Description

3-(4,6-Difluortriazinylamino)-7-Methoxycoumarin is a specialized, reactive fluorescent dye derivative designed for advanced labeling applications. This compound matters because it combines the excellent fluorescent properties of a methoxycoumarin core with a reactive difluorotriazinyl group, enabling efficient covalent conjugation to biomolecules under mild conditions. It is primarily needed by researchers and manufacturers in the life sciences sector for developing sensitive diagnostic assays, fluorescence microscopy probes, and bioanalytical reagents.

Application

• Fluorescent Labeling of Biomolecules: Covalent attachment to proteins, antibodies, peptides, and nucleic acids for detection and analysis.
• Development of Diagnostic Assays: Used as a key component in fluorescence immunoassays (FIA), ELISA, and other clinical diagnostic kits.
• Cellular and Tissue Imaging: Synthesis of probes for fluorescence microscopy (e.g., confocal, super-resolution) to visualize cellular structures and processes.
• Bioconjugation & Chemical Biology: A versatile tool for tagging biomolecules in drug discovery, proteomics, and molecular biology research.
• Material Science Markers: Potential use in labeling polymers or surfaces for tracking and analysis in advanced materials.
• High-Throughput Screening (HTS): Employed in the development of fluorescent sensors and indicators for pharmaceutical screening.

Basic Information

Product Name	3-(4,6-Difluortriazinylamino)-7-Methoxycoumarin
CAS No.	127192-67-0
Molecular Formula	C19H13F2N5O3
Molecular Weight	397.34 g/mol
Synonyms	7-Methoxycoumarin-3-carboxylic acid (4,6-difluoro-1,3,5-triazin-2-yl)amide; 3-[(4,6-Difluoro-1,3,5-triazin-2-yl)amino]-7-methoxy-2H-1-benzopyran-2-one; Amino Coumarin DFTA; Dye 7-MCA-DFTA; Fluorescent Triazine Dye; 7-Methoxycoumarin-3-yl DFTA; Reactive Coumarin 343 derivative; ATTO 425 analogue
EINECS	Contact for details

Quality Control

Our production of 3-(4,6-Difluortriazinylamino)-7-Methoxycoumarin adheres to stringent in-process controls to ensure batch-to-batch consistency and high performance in sensitive applications. Every lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and the absence of critical impurities. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is light-sensitive and should be handled under appropriate conditions to prevent degradation. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Fluorescence Properties	Ex/Em ~385/445 nm (solvent dependent)
Single Spot (TLC)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.