Description

n-Octadecanoyl-Nile Blue is a chemically modified, lipophilic derivative of the classic Nile Blue fluorescent dye, where the hydroxyl group is esterified with a long-chain stearic acid moiety. This structural modification significantly enhances its lipid solubility and membrane permeability, making it a powerful tool for staining and tracking lipids in complex biological systems. It is primarily utilized by researchers and manufacturers in the life sciences, particularly for advanced cellular imaging, lipid droplet visualization, and the development of diagnostic assays.

Application

• Fluorescent Staining of Lipid Droplets: Selective labeling and visualization of neutral lipid stores in adipocytes, hepatocytes, and other cell types for metabolic studies.
• Live-Cell and Fixed-Cell Imaging: Used as a vital stain in fluorescence microscopy (e.g., confocal, epifluorescence) to monitor lipid dynamics in real-time.
• Flow Cytometry Analysis: Enables the quantification and sorting of cell populations based on lipid content.
• Biomedical Research: Investigation of metabolic disorders, such as obesity, fatty liver disease, and atherosclerosis.
• Diagnostic Reagent Development: A key component in formulating kits for the detection and analysis of lipids in clinical and research samples.
• Pharmaceutical Screening: Used in high-throughput assays to evaluate drug candidates that modulate lipid metabolism.

Basic Information

Product Name	n-Octadecanoyl-Nile Blue
CAS No.	125829-24-5
Molecular Formula	C46H65N3O3
Molecular Weight	708.03 g/mol
Synonyms	Stearoyl Nile Blue; Nile Blue Stearate; 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one, 10-octadecanoyl derivative; LipidTOX™ Deep Red (competitive/comparable stain); Lipophilic Nile Blue dye; Fluorescent lipid probe; Nile Blue C18 ester
EINECS	Contact for details

Quality Control

Our n-Octadecanoyl-Nile Blue is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and fluorescence properties. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. For long-term storage, consider keeping the product desiccated under an inert atmosphere to maintain optimal stability and fluorescence yield.

Specification

Item	Specification
Appearance	Dark blue to greenish-blue solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Absorption Max (λmax)	630 - 650 nm
Emission Max (λem)	660 - 680 nm
Solubility	Soluble in DMSO, DMF, ethanol; sparingly soluble in aqueous buffers
Loss on Drying	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.