Description

Acetamidotetramethylrhodamine is a high-purity, fluorescent dye derivative widely used as a critical labeling reagent in advanced life science research. This compound is valued for its excellent photostability and bright fluorescence, enabling highly sensitive detection and imaging applications. It is primarily utilized by researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors for labeling proteins, antibodies, and nucleic acids.

Application

• Fluorescent Labeling of Biomolecules: Conjugation to antibodies, proteins, peptides, and oligonucleotides for use in fluorescence microscopy, flow cytometry, and immunoassays.
• Bioconjugation & Probe Synthesis: Serving as a reactive intermediate for synthesizing custom fluorescent probes and sensors.
• Live-Cell and Fixed-Cell Imaging: Providing a stable, bright signal for tracking cellular processes and localization studies.
• Diagnostic Assay Development: Used as a detection label in the development of ELISA, lateral flow assays, and other in vitro diagnostic (IVD) kits.
• Nucleic Acid Detection: Labeling primers or probes for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Pharmaceutical Research: Tracing drug delivery mechanisms and studying pharmacokinetics in preclinical research models.

Basic Information

Product Name	Acetamidotetramethylrhodamine
CAS No.	124985-63-3
Molecular Formula	C32H31N3O5
Molecular Weight	537.61 g/mol
Synonyms	5-(and-6)-Acetamidotetramethylrhodamine; Acetamido TAMRA; 5/6-ATMR; 5(6)-Carboxy-X-rhodamine, acetamido; N-(5-Carboxypentyl)-5-(and-6)-carboxytetramethylrhodamine, acetamido; TAMRA, acetamido; ATTO 550 equivalent; Rhodamine derivative, acetamido
EINECS	Contact for details

Quality Control

Our Acetamidotetramethylrhodamine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopic identification, and impurity profiling to ensure it meets the stringent requirements of research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, identity, and performance characteristics is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions for long-term stability. For short-term use, the product may be stored at 2-8°C. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Single Isomer Content (HPLC)	≥ 90.0%
Extinction Coefficient	≥ 90,000 cm⁻¹M⁻¹ (at λ max)
Fluorescence Quantum Yield	Contact for details
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF, MeCN

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.