Description

5-Cfda, Am is a specialized fluorogenic substrate and biochemical reagent widely used in cell biology and diagnostic research. Its core value lies in its ability to serve as a vital tool for measuring intracellular esterase activity and cell viability through fluorescence-based assays. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and life sciences sectors who require high-purity biochemicals for advanced cellular analysis and diagnostic kit development.

Application

• Cell Viability and Proliferation Assays: Used as a fluorogenic substrate to measure intracellular esterase activity, a key indicator of live cells.
• Flow Cytometry and Fluorescence Microscopy: Functions as a vital stain for identifying and quantifying viable cell populations in complex samples.
• Diagnostic Kit Manufacturing: A critical raw material in the production of kits for clinical diagnostics and research use only (RUO) applications.
• Toxicology and Drug Screening: Employed in in vitro studies to assess compound cytotoxicity and effects on cell metabolism.
• Microbiology Research: Applied in assays to study microbial viability and enzymatic activity in environmental and clinical isolates.
• Biomarker Detection: Utilized in developing assays for specific enzymatic biomarkers associated with various physiological and pathological states.

Basic Information

Product Name	5-Cfda, Am
CAS No.	124412-00-6
Molecular Formula	C25H18F3NO6
Molecular Weight	485.41 g/mol
Synonyms	5-Carboxyfluorescein Diacetate, Acetoxymethyl Ester; CFDA, AM ester; 5(6)-CFDA, AM; 5-CFDA, Acetoxymethyl Ester; CFDA-AM; 5-Carboxyfluorescein Diacetate Acetoxymethyl Ester; Cell Viability Stain; Fluorescein Diacetate Derivative
EINECS	Contact for details

Quality Control

Our 5-Cfda, Am is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biological applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange-yellow solid
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.