Description

5(6)-Cfda CAS NO 124387-19-5 is a high-purity, fluorescent dye derivative of carboxyfluorescein diacetate, specifically designed as a vital, non-toxic cell tracer for live-cell imaging and flow cytometry. This compound is critical for researchers and manufacturers requiring a reliable, cell-permeant probe that is enzymatically converted to a fluorescent, cell-impermeant product, enabling long-term tracking of cell viability, proliferation, and migration. It is an essential tool for biotechnology, pharmaceutical development, and academic research focused on cell biology, immunology, and high-throughput screening applications.

Application

• Live-Cell Fluorescent Labeling: Used as a cell-permeant tracer for non-invasive, long-term monitoring of cell populations in culture.
• Cell Viability & Proliferation Assays: Enzymatic conversion within live cells provides a fluorescent signal proportional to esterase activity and cell health.
• Flow Cytometry Analysis: A standard dye for staining cells to analyze viability, count, and sort live cell populations.
• Cell Migration & Tracking Studies: Enables the tracing of cell movement and localization in wound healing, chemotaxis, and developmental biology models.
• Cytotoxicity Testing: Serves as an indicator in assays screening for drug or compound toxicity based on the loss of intracellular esterase activity.
• High-Content Screening (HCS): Utilized in automated imaging platforms for phenotypic screening in drug discovery.

Basic Information

Product Name	5(6)-Cfda
CAS No.	124387-19-5
Molecular Formula	C25H16O9
Molecular Weight	460.39 g/mol
Synonyms	5(6)-Carboxyfluorescein Diacetate; 5(6)-CFDA; CFDA; Carboxyfluorescein Diacetate, Mixed Isomers; 3',6'-Diacetylfluorescein-5(6)-carboxylic Acid; 5(6)-Carboxyfluorescein Diacetate, Mixed Isomers; CFDA, SE (precursor); CellTrace™ CFDA (related commercial name).
EINECS	Contact for details

Quality Control

Every batch of 5(6)-Cfda is manufactured and tested under strict quality management systems to ensure consistency and performance for critical research applications. Our products undergo rigorous quality testing, including HPLC for purity and identity confirmation, to ensure compliance with high-grade standards suitable for cell-based assays. A comprehensive Certificate of Analysis (COA) detailing purity, fluorescence performance, and absence of impurities is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is light-sensitive and should be handled under appropriate conditions to prevent degradation. For long-term storage, desiccate at -20°C.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Fluorescence Properties	Ex/Em ~492/517 nm (upon hydrolysis)
Solubility	Soluble in DMSO, DMF
Loss on Drying	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.