Description

Xylene Cyanol is a synthetic triphenylmethane dye widely used as a tracking dye in molecular biology and biochemistry. Its distinct blue color and predictable migration rate make it an essential tool for monitoring the progress of nucleic acid electrophoresis. This high-purity compound is critical for researchers and technicians requiring precise and reliable performance in analytical procedures. It is a key material for laboratories in the pharmaceutical, biotechnology, and academic research sectors.

Application

• Electrophoresis Tracking Dye: Primarily used in agarose and polyacrylamide gel electrophoresis (PAGE) to visualize the migration front of DNA and RNA samples.
• Molecular Biology Reagents: A standard component in loading dyes for DNA sequencing, PCR product analysis, and restriction enzyme digestion verification.
• Protein Electrophoresis: Sometimes employed in SDS-PAGE as a visible marker for tracking the leading edge of protein samples during separation.
• Laboratory Staining: Can be used as a colorimetric stain in certain histochemical and cytological applications.
• Quality Control Marker: Serves as a reference standard in the production and quality control of other electrophoresis reagents and kits.

Basic Information

Product Name	Xylene Cyanol
CAS No.	124262-82-4
Molecular Formula	C25H27N2NaO6S2
Molecular Weight	538.61 g/mol
Synonyms	Acid Blue 147; C.I. 42135; Xylene Cyanol FF; Xylene Cyanol FCF; Xylene Cyanol A; XC; Brilliant Acid Blue BS; Acid Brilliant Blue BS; Sodium 4-[[4-(ethyl)(3-sulfophenyl)methyl]phenyl][4-(ethyl)(4-sulfophenyl)methylidene]cyclohexa-2,5-dien-1-ylidene]-N-ethyl-3-sulfoanilinate
EINECS	Contact for details

Quality Control

Our Xylene Cyanol is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot is tested to meet stringent purity criteria suitable for sensitive molecular biology applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity (by HPLC), and other critical parameters is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at a controlled room temperature (15-25°C) in a dry, well-ventilated area. Keep the container tightly sealed when not in use to protect from moisture.

Specification

Item	Specification
Appearance	Dark blue to blue-black powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Loss on Drying	≤ 5.0 %
Absorbance Profile	Conforms to standard
Electrophoresis Performance	Suitable for use

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.