Description

4-Azido-2-Nitrophenyl Pyrophosphate is a specialized biochemical reagent featuring a photoreactive azido group and a high-energy pyrophosphate moiety. This compound matters as a critical tool for photoaffinity labeling and cross-linking studies, enabling researchers to probe enzyme active sites and map protein-nucleotide interactions. It is primarily needed by research institutions and pharmaceutical companies engaged in enzymology, structural biology, and the development of nucleotide-based therapeutics.

Application

• Photoaffinity Labeling Probes: Acts as a key reagent for covalently tagging and identifying nucleotide-binding sites on enzymes and receptors upon UV irradiation.
• Enzyme Mechanism Studies: Used to investigate the catalytic mechanisms of kinases, polymerases, and other pyrophosphate-utilizing enzymes.
• Structural Biology: Facilitates the study of protein structure and dynamics by creating irreversible cross-links for analysis via mass spectrometry or crystallography.
• Bioconjugation & Probe Synthesis: Serves as a building block for synthesizing advanced molecular probes and diagnostic tools.
• Biochemical Research Reagent: Employed in fundamental research to understand signal transduction pathways involving nucleotide derivatives.

Basic Information

Item	Details
Product Name	4-Azido-2-Nitrophenyl Pyrophosphate
CAS No.	123883-88-5
Molecular Formula	C12H8N5O11P2
Molecular Weight	459.10 g/mol
Synonyms	ANPP; 2-Nitro-4-azidophenyl pyrophosphate; 4-Azido-2-nitrophenyl diphosphate; P1,P2-Bis(4-azido-2-nitrophenyl) diphosphate; Photoreactive pyrophosphate analog; Azidophenyl pyrophosphate ester
EINECS	Contact for details

Quality Control

Our 4-Azido-2-Nitrophenyl Pyrophosphate is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to ISO 9001 quality management standards, and our analytical methods, including HPLC and NMR, are validated to provide reliable specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is light-sensitive (store away from light) and hygroscopic (moisture-sensitive). For long-term stability, store under inert atmosphere. Allow the container to reach room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	Yellow to orange powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.