Description

Fluo 3 CAS NO 123632-39-3 is a high-purity, cell-permeant fluorescent calcium indicator widely used in biological research. This compound is essential for the quantitative measurement of intracellular calcium ion (Ca²⁺) concentrations in live cells, providing critical insights into cellular signaling pathways. It is a vital tool for researchers and scientists in the fields of neuroscience, cardiology, pharmacology, and cell biology who require precise and reliable fluorescent probes for confocal microscopy, flow cytometry, and fluorescence plate reader assays.

Application

• Intracellular Calcium Imaging: Primary application for real-time visualization and quantification of cytosolic Ca²⁺ transients in live cells.
• Neuroscience Research: Studying calcium signaling in neurons, synaptic transmission, and neuronal activity.
• Cardiovascular Research: Monitoring calcium flux in cardiomyocytes to understand heart muscle contraction and related pathologies.
• Drug Discovery & Pharmacology: High-throughput screening (HTS) assays to evaluate the effect of pharmaceutical compounds on cellular calcium homeostasis.
• Cell Biology Studies: Investigating calcium's role in processes like apoptosis, cell cycle progression, and enzyme activation.
• Fluorescence-Activated Cell Sorting (FACS): Used as a fluorescent marker for analyzing and sorting cell populations based on calcium levels.

Basic Information

Product Name	Fluo 3
CAS No.	123632-39-3
Molecular Formula	C₅₁H₅₀Cl₂N₂O₂₃
Molecular Weight	1129.84 g/mol
Synonyms	Fluo-3; Fluo 3 AM; Fluo-3 AM ester; Fluo-3, acetoxymethyl ester; Fluo-3, pentapotassium salt; 2-[6-[Bis(carboxymethyl)amino]-5-(2,7-dichloro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]methyl]-8-[bis(carboxymethyl)amino]quinoline; 2-[6-[Bis(2-carboxyethyl)amino]-5-(2,7-dichloro-6-hydroxy-3-oxo-9H-xanthen-9-yl)-2-benzofuranyl]-5-oxazolecarboxylic acid, AM ester
EINECS	Contact for details

Quality Control

Our Fluo 3 is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance in sensitive biological assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic analysis for identity confirmation. We provide a detailed Certificate of Analysis (COA) with every shipment, documenting key parameters such as purity, fluorescence properties, and absence of critical impurities. Our quality system is designed to meet the rigorous standards required for life science research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Fluorescence Properties	Ex/Em ~506/526 nm (Ca²⁺ bound)
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.