Description

Alcian Blue Pyridine Variant is a specialized cationic phthalocyanine dye, a variant of the classic Alcian Blue 8GX, where the pyridine groups are integral to its structure and staining properties. This compound is critically important for its high specificity in histochemical and cytochemical staining, particularly for the visualization of acidic mucopolysaccharides and glycoproteins. It is an essential reagent for researchers and diagnostic laboratories in life sciences, pathology, and biomedical research, where precise cellular and tissue analysis is required.

Application

• Histopathological Staining: Primary application for staining acidic sulfated and carboxylated mucins, mucopolysaccharides, and glycoproteins in tissue sections.
• Cytological Analysis: Used in cytology smears and cell cultures to differentiate cell types based on secretory product composition.
• Cartilage and Connective Tissue Research: Essential for visualizing proteoglycans in cartilage matrix and other connective tissues in research models.
• Microbiological Staining: Can be employed for staining bacterial capsules and certain fungal cell wall components.
• Electron Microscopy: Sometimes used as a stain for ultrastructural studies, often in combination with other reagents.
• Quality Control in Biologics: Used in the characterization of glycosaminoglycan content in biopharmaceutical products.
• Teaching and Diagnostics: A standard dye in educational histology kits and routine diagnostic pathology panels (e.g., Alcian Blue/PAS stain).

Basic Information

Product Name	Alcian Blue Pyridine Variant
CAS No.	123439-83-8
Molecular Formula	C56H68Cl4CuN16S4
Molecular Weight	1299.86 g/mol
Synonyms	Alcian Blue 8GX Pyridine Variant; Ingrain Blue 1; C.I. 74240; Copper Phthalocyanine Tetramethyl Derivative; Alcian Blue Tetrakis(methylpyridinium) Chloride; Astra Blue; Astrablau; Alcianblau 8GX
EINECS	Contact for details

Quality Control

Our Alcian Blue Pyridine Variant is manufactured and tested to ensure consistent performance for critical histological applications. Each batch is subjected to rigorous analytical controls, including spectrophotometric purity assessment, thin-layer chromatography (TLC), and functional staining validation against reference standards. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and performance specifications is provided with every shipment to guarantee traceability and compliance with research-grade quality standards.

Storage

Preserve in a tightly closed container, protected from light. Store at a controlled room temperature (15-25°C) in a dry environment. The product is light-sensitive; prolonged exposure to light may affect staining performance.

Specification

Item	Specification
Appearance	Dark blue to greenish-blue powder
Identification (IR)	Conforms to structure
Identification (TLC)	Single major spot
Absorbance (Max)	~610-620 nm (in aqueous solution)
Functional Staining	Passes performance test vs. control tissue
Loss on Drying	≤ 5.0 %
Heavy Metals (as Pb)	≤ 20 ppm
Solubility	Soluble in water, slightly soluble in alcohols

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.