Description

Methylene Blue CAS NO 122965-43-9 is a synthetic heterocyclic aromatic compound and a vital cationic dye belonging to the phenothiazine class. It serves as a versatile chemical with significant utility across scientific, medical, and industrial sectors due to its redox-active properties and distinctive color. This product is essential for professionals in pharmaceutical research, microbiology, histology, and water treatment who require a reliable and high-purity reagent for critical applications.

Application

• Biological Staining: Widely used as a vital stain in microscopy for cell nuclei, bacteria (e.g., in Gram staining), and blood films (e.g., for malaria parasites).
• Medical & Pharmaceutical: Employed as an antidote for methemoglobinemia and as a surgical marker dye; also investigated for its potential in photodynamic therapy and antiviral research.
• Analytical Chemistry: Functions as a redox indicator in titrations and as a reagent in various biochemical assays to measure cellular respiration and reducing equivalents.
• Aquaculture & Aquarium Industry: Used as a treatment for fungal and parasitic infections in fish and fish eggs.
• Industrial Dyeing: Applied in the textile and paper industries for coloring cotton, silk, and paper, and in the creation of inks.
• Water Treatment & Research: Acts as a tracer dye in hydrology and environmental studies, and is used in research on advanced oxidation processes.

Basic Information

Item	Details
Product Name	Methylene Blue
CAS No.	122965-43-9
Molecular Formula	C16H18ClN3S
Molecular Weight	319.85 g/mol
Synonyms	Methylthioninium Chloride; Basic Blue 9; CI 52015; Swiss Blue; Urolene Blue; Methylenum Caeruleum; 3,7-Bis(Dimethylamino)phenothiazin-5-ium Chloride; Tetramethylthionine Chloride
EINECS	Contact for details

Quality Control

Our Methylene Blue is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including identification, purity assay, and impurity profiling, to ensure it meets exacting standards. A Certificate of Analysis (COA) detailing all test results is provided with every shipment to guarantee traceability and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). Keep away from incompatible materials.

Specification

Item	Specification
Appearance	Dark green crystalline powder with bronze luster
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition (Sulfated Ash)	≤ 0.1%
Heavy Metals (as Pb)	≤ 20 ppm
Water Solubility	Completely soluble

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.