Description

Tetramethylrhodamine Succinimidyl Ester is a high-purity, reactive fluorescent dye derivative essential for advanced biomolecular labeling and detection. This compound is a critical reagent for creating stable, covalent conjugates with primary amines, enabling highly sensitive analysis in complex biological systems. It is primarily utilized by researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors for applications such as flow cytometry, fluorescence microscopy, and immunoassay development.

Application

• Protein and Antibody Labeling: Covalent conjugation to primary amine groups (e.g., lysine residues) for fluorescence-activated cell sorting (FACS) and Western blotting.
• Nucleic Acid Probe Synthesis: Labeling of oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR detection.
• Cell Tracing and Tracking: Long-term fluorescent labeling of cells for viability, proliferation, and migration studies in live-cell imaging.
• Diagnostic Assay Development: Key component in the manufacture of fluorescent lateral flow assays and ELISA kits for high-sensitivity detection.
• Biomaterial and Nanoparticle Functionalization: Surface modification of polymers, dendrimers, and nanoparticles for targeted imaging and drug delivery research.
• Receptor-Ligand Binding Studies: Tagging of peptides, proteins, or small molecules to visualize and quantify binding interactions.

Basic Information

Product Name	Tetramethylrhodamine Succinimidyl Ester
CAS No.	122363-35-3
Molecular Formula	C29H27N3O7
Molecular Weight	529.55 g/mol
Synonyms	5(6)-TAMRA, SE; 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester; 6-TAMRA, SE; Tetramethylrhodamine-5(6)-isomer N-succinimidyl ester; TAMRA NHS ester; 5(6)-TAMRA, NHS ester; NSC 113927; 6-Carboxy-tetramethylrhodamine succinimidyl ester
EINECS	Contact for details

Quality Control

Our Tetramethylrhodamine Succinimidyl Ester is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopy. Certificates of Analysis (COA) detailing all specifications are provided and can be tailored to meet specific research or GMP-grade requirements upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize condensation and hydrolysis.

Specification

Item	Specification
Appearance	Red to purple solid
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 95.0%
Single Isomer Content (HPLC)	≥ 90.0% (5- or 6-isomer)
Extinction Coefficient (ε)	Contact for details
Fluorescence Emission (λmax)	~575 nm
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.