Description

Carzelesin is a synthetic antineoplastic agent belonging to the duocarmycin analog class, designed for targeted DNA alkylation in preclinical oncology research. It offers high potency and sequence-selective cytotoxicity, making it a critical tool for mechanistic studies in cancer drug discovery and development. This compound is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and contract research organizations (CROs) engaged in anticancer agent screening and structure–activity relationship (SAR) analysis.

Application

• Preclinical evaluation of DNA-alkylating agents in solid tumor and hematologic malignancy models
• Lead optimization studies for next-generation duocarmycin-based prodrugs and antibody–drug conjugates (ADCs)
• Mechanistic investigation of minor-groove binding, DNA adduct formation, and apoptotic signaling pathways
• Biochemical assay development for high-throughput screening of DNA-damaging compounds
• Reference standard in analytical method validation (HPLC, LC-MS) for cytotoxic small molecules
• Pharmacokinetic and metabolism studies in rodent and non-rodent species
• Stability-indicating method development under stressed conditions (e.g., light, humidity)
• Comparative cytotoxicity profiling across human cancer cell lines (e.g., NCI-60 panel)

Basic Information

Product Name	Carzelesin
CAS No.	119813-10-4
Molecular Formula	C35H37ClN4O7
Molecular Weight	661.15 g/mol
Synonyms	NSC 655649; U-80244; (1R,2S,3R,4S)-1-(Acetyloxy)-2-[(chloroacetyl)amino]-3-(methoxycarbonyl)-4-[(2-methyl-1H-indol-3-yl)carbonyl]cyclopentane; Duocarmycin SA analog; Carzelesin hydrochloride (common salt form); 1,2,3,4-Tetrahydro-1-(acetyloxy)-2-[(chloroacetyl)amino]-3-(methoxycarbonyl)-4-[(2-methyl-1H-indol-3-yl)carbonyl]cyclopentane; U 80244; NSC-655649
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. All batches of Carzelesin are tested for identity (IR, HPLC, NMR), assay (HPLC), related substances (HPLC), residual solvents (GC), and heavy metals (USP <231>). Testing adheres to ICH Q2(R2) guidelines and supports GMP-aligned research environments.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, maintain low-humidity conditions (<30% RH) and minimize exposure to ambient air during handling.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.0%
Related Substances (HPLC)	Single impurity ≤0.5%; Total impurities ≤2.0%
Residual Solvents (GC)	Meets ICH Q3C limits
Water (Karl Fischer)	≤0.5%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.