Description

4',5'-Dimethylfluorescein is a chemically modified derivative of fluorescein, a prominent xanthene dye. This compound is valued for its role as a key synthetic intermediate and a specialized fluorescent probe in advanced research and analytical chemistry. It is primarily utilized by professionals in the pharmaceutical, life sciences, and fine chemical sectors. The product is supplied with a CAS registry number of 118797-71-0.

Application

• Fluorescent Probe Development: Serves as a precursor for synthesizing advanced fluorescent dyes and molecular probes with tailored spectral properties.
• Bioconjugation & Labeling: Used in the preparation of labeled antibodies, proteins, and nucleotides for fluorescence microscopy and flow cytometry.
• Sensor Chemistry: Acts as a core structure in the design of chemosensors for detecting metal ions, pH changes, or specific biomolecules.
• Organic Synthesis Intermediate: A crucial building block for the synthesis of more complex xanthene-based dyes and pharmaceutical compounds.
• Analytical Reagent: Employed as a standard or reagent in high-performance liquid chromatography (HPLC) and other analytical methods.
• Material Science Research: Investigated for potential applications in developing fluorescent polymers and advanced optical materials.

Basic Information

Product Name	4',5'-Dimethylfluorescein
CAS No.	118797-71-0
Molecular Formula	C24H18O5
Molecular Weight	386.40 g/mol
Synonyms	4',5'-Dimethylfluorescein; 2-(3',6'-Dihydroxy-4',5'-dimethyl-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-6-yl)benzoic acid; 4',5'-Dimethyl-3',6'-dihydroxyfluoran; 4',5'-Dimethylfluorone; Fluorescein, 4',5'-dimethyl-; 3',6'-Dihydroxy-4',5'-dimethylfluoran
EINECS	Contact for details

Quality Control

Our 4',5'-Dimethylfluorescein is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and industrial use. A detailed Certificate of Analysis (COA) is provided with every shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.