Description

Fluorescein-5-Isothiocyanate Diacetate is a high-purity, protected derivative of fluorescein isothiocyanate (FITC), a cornerstone fluorescent labeling reagent. This diacetate form offers enhanced stability and solubility for specific conjugation protocols, making it a critical intermediate for synthesizing advanced fluorescent probes. It is essential for researchers and manufacturers in the life sciences and diagnostics industries who require reliable, high-quality reagents for protein, antibody, and nucleic acid labeling applications.

Application

• Fluorescent Probe Synthesis: A key precursor for creating custom fluorescent conjugates and molecular beacons.
• Biomolecule Labeling: Used in the preparation of fluorescently-labeled antibodies, proteins, peptides, and oligonucleotides for assays like flow cytometry (FACS) and immunofluorescence (IF).
• Diagnostic Kit Manufacturing: Serves as a critical raw material in the production of fluorescent-based in-vitro diagnostic (IVD) kits and lateral flow assays.
• Cell Biology Research: Enables the study of cellular processes, receptor binding, and intracellular trafficking through fluorescence microscopy.
• Bioconjugation Chemistry: Provides a reactive isothiocyanate group (-N=C=S) for stable thiourea bond formation with primary amines under mild conditions.
• Material Science: Used to functionalize nanoparticles and polymers with fluorescent tags for tracking and imaging applications.

Basic Information

Product Name	Fluorescein-5-Isothiocyanate Diacetate
CAS No.	118378-76-0
Molecular Formula	C27H17NO8S
Molecular Weight	515.49 g/mol
Synonyms	5-FITC Diacetate; Fluorescein 5-Isothiocyanate Diacetate; FITC-5-Diacetate; 5-Isothiocyanatofluorescein Diacetate; 5-(Isothiocyanato)fluorescein Diacetate; 2',7'-Diacetyl-6-isothiocyanatofluorescein; 6-Isothiocyanatofluorescein Diacetate; Fluorescein Diacetate-5-Isothiocyanate
EINECS	Contact for details

Quality Control

Our Fluorescein-5-Isothiocyanate Diacetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available for every lot upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, DMF, acetone
Loss on Drying	≤ 1.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.