Description

6-Carboxy-X-Rhodamine Succinimidyl Ester is a highly reactive, amine-reactive fluorescent labeling reagent widely used in life science research. This compound is critical for creating stable, fluorescent conjugates with proteins, antibodies, peptides, and other biomolecules for sensitive detection and imaging. It is an essential tool for researchers and manufacturers in biotechnology, diagnostics, and pharmaceutical development who require bright, photostable labeling for applications like flow cytometry, fluorescence microscopy, and immunoassays.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to primary amines (-NH₂) on antibodies, proteins, peptides, and oligonucleotides for detection.
• Flow Cytometry & Cell Analysis: Preparation of fluorescent antibody probes for cell surface marker detection and intracellular staining.
• Fluorescence Microscopy & Imaging: Creating targeted imaging agents for cellular and tissue visualization in research.
• Immunoassay Development: Labeling detection antibodies in ELISA, Western blotting, and other immunoassay platforms.
• Diagnostic Kit Manufacturing: Used in the production of fluorescent-based in vitro diagnostic (IVD) reagents and kits.
• Bioconjugation & Probe Synthesis: A key building block in the synthesis of custom fluorescent probes and molecular beacons.

Basic Information

Item	Detail
Product Name	6-Carboxy-X-Rhodamine Succinimidyl Ester
CAS No.	117491-83-5
Molecular Formula	C₃₄H₃₀N₂O₁₀
Molecular Weight	626.61 g/mol
Synonyms	6-ROX NHS Ester; 6-Carboxy-X-rhodamine, succinimidyl ester; X-Rhodamine-6-COOH NHS Ester; 6-Carboxy-X-rhodamine N-hydroxysuccinimide ester; 6-ROX, SE; 6-Carboxy-X-rhodamine, N-succinimidyl ester; 6-Carboxy-X-rhodamine, NHS ester; 6-Carboxy-X-rhodamine, SE
EINECS	Contact for details

Quality Control

Our 6-Carboxy-X-Rhodamine Succinimidyl Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for labeling efficiency verification, and identity confirmation. We provide Certificates of Analysis (COA) with each shipment, detailing all relevant specifications to ensure batch-to-batch consistency and performance for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, keep under inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~575 nm
Emission Maximum (λem)	~602 nm
Extinction Coefficient (ε)	Contact for details
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.