Description

Fluorescein Isothiocyanate-Phenylglyoxal is a specialized bifunctional fluorescent labeling reagent. This compound is engineered for the selective conjugation and detection of biomolecules, combining the high fluorescence yield of fluorescein with the arginine-specific reactivity of phenylglyoxal. It is essential for researchers and manufacturers requiring precise, dual-mechanism tagging in complex biological systems. Key applications span advanced biochemical research, diagnostic assay development, and high-sensitivity protein analysis.

Application

• Protein Labeling & Conjugation: Used for the fluorescent tagging of proteins, particularly targeting arginine residues for structure-function studies.
• Biomolecular Assay Development: Serves as a critical reagent in developing fluorescence-based immunoassays (FLISA) and other diagnostic tests.
• Cell Biology & Microscopy: Employed in fluorescence microscopy and flow cytometry for visualizing cellular components and tracking biological processes.
• Bioconjugation Chemistry: Acts as a versatile linker for attaching fluorescent probes to antibodies, peptides, and other ligands.
• Proteomics Research: Facilitates the analysis of protein-protein interactions, post-translational modifications, and enzyme activity.
• Diagnostic Kit Manufacturing: Integrated into the production of high-sensitivity commercial diagnostic kits for clinical and research markets.

Basic Information

Product Name	Fluorescein Isothiocyanate-Phenylglyoxal
CAS No.	116843-39-1
Molecular Formula	C23H14N2O5S
Molecular Weight	430.44 g/mol
Synonyms	FITC-Phenylglyoxal; 5-Isothiocyanatofluorescein Phenylglyoxal; Phenylglyoxal-FITC; Fluorescein 5-Isothiocyanate Phenylglyoxal Adduct; 2-Oxo-2-phenylacetaldehyde Fluorescein Isothiocyanate Derivative; [5-(Isothiocyanato)-9H-xanthen-9-ylidene]phenylacetaldehyde; Reactive Fluorescein-Arginine Labeling Reagent
EINECS	Contact for details

Quality Control

Every batch of Fluorescein Isothiocyanate-Phenylglyoxal is manufactured under strict quality management systems. Our products undergo rigorous quality testing, including HPLC for purity and identity verification, to ensure compliance with high-purity research and diagnostic standards. Certificates of Analysis (COA) with lot-specific data are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed under inert atmosphere or with desiccant after each use to maintain stability and performance.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Fluorescence Emission	Characteristic of FITC (~520 nm)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.