Description

Tetramethylrhodamine Methyl Ester Perchlorate is a high-purity, cell-permeant fluorescent dye and vital tool for life science research. This compound is a cationic, red-orange fluorescent probe specifically designed for labeling mitochondria in live cells, making it essential for studies of mitochondrial membrane potential, cell viability, and apoptosis. Researchers in biotechnology, pharmaceutical development, and academic institutions rely on its consistent performance for flow cytometry, fluorescence microscopy, and other advanced cellular assays.

Application

• Mitochondrial Staining: Primary application for visualizing and assessing mitochondrial membrane potential (δψm) in live cells.
• Apoptosis & Cell Viability Assays: Used as a key indicator in assays monitoring early stages of programmed cell death and overall cell health.
• Flow Cytometry: Enables quantitative analysis of mitochondrial function and cell population studies using red-orange fluorescence channels.
• Fluorescence Microscopy: Provides high-contrast, specific labeling of mitochondria for real-time imaging and time-lapse studies.
• Neurobiology Research: Applied in studies of neuronal metabolism, bioenergetics, and mitochondrial dynamics.
• Cancer Research: Utilized to investigate metabolic shifts, drug-induced toxicity, and mitochondrial dysfunction in cancer cell lines.
• Toxicology Screening: Serves as a sensitive probe for assessing compound-induced mitochondrial toxicity in vitro.

Basic Information

Product Name	Tetramethylrhodamine Methyl Ester Perchlorate
CAS No.	115532-50-8
Molecular Formula	C26H26ClN2O7
Molecular Weight	513.95 g/mol
Synonyms	TMRM; Tetramethylrhodamine Methyl Ester; TMRM Perchlorate; TMRE; Tetramethylrhodamine Ethyl Ester (related analog); Rhodamine 6G Methyl Ester Perchlorate; 2-(6-(Dimethylamino)-3-(dimethyliminio)-3H-xanthen-9-yl)benzoate methyl ester perchlorate; Mitochondrial Membrane Potential Probe TMRM
EINECS	Contact for details

Quality Control

Our Tetramethylrhodamine Methyl Ester Perchlorate is manufactured under strict quality systems to ensure batch-to-batch consistency critical for sensitive biological assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting key specifications such as assay purity, absorbance/fluorescence profiles, and moisture content. We support research and GLP-compliant environments with full traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Red to purple crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Absorbance (λmax)	548 nm ± 2 nm (in methanol)
Emission (λmax)	573 nm ± 5 nm (in methanol)
Extinction Coefficient	~95,000 cm-1M-1
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.