Description

n-Hydroxysuccinimidyl Pyrenebutanoate is a high-purity, amine-reactive fluorescent labeling reagent. This compound is specifically designed for the covalent attachment of pyrene fluorophores to primary amines in biomolecules, enabling sensitive detection and analysis. It is an essential tool for researchers and manufacturers in the fields of bioconjugation, proteomics, and diagnostic assay development, where reliable and efficient fluorescent tagging is required.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation to primary amine groups (e.g., lysine residues) for fluorescence microscopy, flow cytometry, and immunoassays.
• Peptide Tagging and Modification: Introduction of a pyrene moiety to study protein-protein interactions, folding, or as a spectroscopic probe.
• Development of Fluorescent Biosensors: Creating labeled substrates or probes for enzymatic activity assays and diagnostic tests.
• Polymer and Material Science Functionalization: Labeling amine-containing polymers, dendrimers, or nanoparticles for tracking and characterization.
• DNA/RNA Probe Synthesis: Modification of amine-modified oligonucleotides for fluorescence in situ hybridization (FISH) and other nucleic acid detection techniques.
• Crosslinking and Bioconjugate Chemistry: Used as a building block in the synthesis of more complex heterobifunctional crosslinkers.

Basic Information

Product Name	n-Hydroxysuccinimidyl Pyrenebutanoate
CAS No.	114932-60-4
Molecular Formula	C25H21NO5
Molecular Weight	415.44 g/mol
Synonyms	Pyrenebutyric Acid N-Hydroxysuccinimide Ester; 1-Pyrenebutanoic Acid N-Hydroxysuccinimide Ester; 1-Pyrenebutyric Acid N-Succinimidyl Ester; NHS-Pyrenebutyrate; Pyrenebutanoic Acid Succinimidyl Ester; Succinimidyl Pyrenebutyrate; 4-(1-Pyrenyl)butyric Acid N-Hydroxysuccinimide Ester; N-(1-Pyrenebutyryloxy)succinimide
EINECS	Contact for details

Quality Control

Our n-Hydroxysuccinimidyl Pyrenebutanoate is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and spectrophotometric analysis. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange powder or crystals
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 95%
Extinction Coefficient	~40,000 cm⁻¹M⁻¹ at 340 nm (in DMSO)
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.