Description

5(6)-Carboxy-X-Rhodamine-n-Hydroxysuccinimide Ester is a high-purity, amine-reactive fluorescent labeling reagent widely used in life science research. This compound is critical for creating stable, fluorescent conjugates with proteins, antibodies, and other biomolecules for sensitive detection and imaging applications. It is an essential tool for researchers and manufacturers in the fields of flow cytometry, immunofluorescence, and nucleic acid analysis, where reliable and bright labeling is paramount.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation to primary amines (e.g., lysine residues) for use in immunoassays (ELISA, Western Blot) and immunohistochemistry.
• Flow Cytometry & Cell Sorting: Preparation of fluorescent antibody probes for cell surface marker detection and analysis.
• Nucleic Acid Labeling: Modification of amine-modified oligonucleotides for fluorescence in situ hybridization (FISH) and microarray applications.
• Biomolecular Imaging: Creation of probes for confocal and super-resolution microscopy to visualize cellular structures and processes.
• Diagnostic Kit Manufacturing: Key raw material in the production of fluorescent lateral flow assays and other in vitro diagnostic devices.
• Drug Discovery & High-Throughput Screening (HTS): Labeling of target molecules or assay components for fluorescence-based detection in screening platforms.

Basic Information

Product Name	5(6)-Carboxy-X-Rhodamine-n-Hydroxysuccinimide Ester
CAS No.	114616-32-9
Molecular Formula	C34H31N3O9
Molecular Weight	625.63 g/mol
Synonyms	5(6)-ROX NHS Ester; 5(6)-Carboxy-X-rhodamine, succinimidyl ester; X-Rhodamine NHS Ester; ROX, SE; 6-Carboxy-X-rhodamine N-succinimidyl ester; 5-Carboxy-X-rhodamine N-succinimidyl ester; 5(6)-Carboxy-X-rhodamine N-hydroxysuccinimide ester; CXR NHS Ester
EINECS	Contact for details

Quality Control

Every batch of our 5(6)-Carboxy-X-Rhodamine-n-Hydroxysuccinimide Ester is manufactured and tested under strict quality management systems. We guarantee high chemical purity and consistent performance for your critical labeling applications. Comprehensive analytical data, including HPLC purity, absorbance/fluorescence spectra, and functional performance testing, is documented in the batch-specific Certificate of Analysis (COA), available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	575 - 585 nm
Emission Maximum	595 - 605 nm
Extinction Coefficient (ε)	≥ 75,000 cm⁻¹M⁻¹
Solubility	Soluble in DMF, DMSO, acetonitrile
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.