Description

Tetramethylrhodamine-5-Iodoacetamidedihydroiodide (5-TMRIA) is a high-purity, reactive fluorescent dye derivative used for site-specific labeling of thiol groups in proteins and other biomolecules. Its core value lies in providing a bright, photostable red-orange fluorescent signal, enabling sensitive detection and analysis in advanced life science research. This compound is essential for researchers and developers in bioconjugation, proteomics, and fluorescence microscopy who require reliable, high-performance probes for tracking molecular interactions and cellular processes.

Application

• Bioconjugation and Protein Labeling: Covalent attachment to cysteine residues (-SH groups) in proteins, antibodies, and peptides for fluorescence tagging.
• Fluorescence Microscopy & Imaging: Used as a fluorescent probe in super-resolution microscopy (e.g., STORM, PALM) and live-cell imaging due to its excellent photophysical properties.
• Flow Cytometry & FACS: Labeling of cell surface proteins or intracellular targets for analysis and sorting via fluorescence-activated cell sorting.
• Biomolecular Assay Development: Serving as a critical component in FRET (Förster Resonance Energy Transfer) assays, biosensors, and diagnostic test development.
• Proteomics Research: Tagging of specific proteins for purification, tracking, or interaction studies within complex biological mixtures.
• Drug Discovery & Screening: Utilization in high-throughput screening (HTS) assays to monitor enzyme activity, binding events, or cellular responses.

Basic Information

Product Name	Tetramethylrhodamine-5-Iodoacetamidedihydroiodide (5-TMRIA)
CAS No.	114458-99-0
Molecular Formula	C25H23IN2O3 · 2HI
Molecular Weight	~ 808.91 g/mol (Free base: 526.38)
Synonyms	5-TMRIA; 5-Tetramethylrhodamine iodoacetamide; TMR-5-IA; 5-Carboxytetramethylrhodamine iodoacetamide; 6-Iodoacetamidotetramethylrhodamine; Rhodamine 6G iodoacetamide derivative; 5-Iodoacetamidotetramethylrhodamine; TAMRA-5-IA
EINECS	Contact for details

Quality Control

Our Tetramethylrhodamine-5-Iodoacetamidedihydroiodide (5-TMRIA) is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance metrics. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the stringent requirements of research and diagnostic-grade chemical synthesis.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant (e.g., in a sealed bag with silica gel) due to its hygroscopic nature. For long-term stability, store the product in a freezer. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with molecular structure
Purity (HPLC)	≥ 95% (Area % at specified wavelength)
Water Content (KF)	≤ 2.0 %
Functional Activity	Successful labeling of standard thiol-containing protein confirmed by fluorescence
Extinction Coefficient	Contact for details
Fluorescence Quantum Yield	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.