Description

Fura 2 Pentapotassium Salt is a high-purity, cell-permeant fluorescent calcium indicator essential for intracellular calcium ([Ca²⁺]i) measurement. Its core value lies in its high affinity for calcium ions and the significant shift in its excitation spectrum upon binding, enabling precise ratiometric quantification crucial for eliminating artifacts. This compound is a fundamental research tool for scientists and professionals in cell biology, neuroscience, pharmacology, and cardiovascular research who require accurate and reliable monitoring of dynamic calcium signaling in live cells.

Application

• Intracellular Calcium Imaging: Primary application for real-time visualization and quantification of cytosolic calcium concentration changes in live cells using fluorescence microscopy or flow cytometry.
• Neuroscience Research: Studying calcium dynamics in neurons and glial cells related to synaptic transmission, neurotransmitter release, and neuronal excitability.
• Cardiovascular Research: Monitoring calcium transients in cardiomyocytes to investigate cardiac muscle contraction, arrhythmias, and drug effects on heart cells.
• Pharmacology & Drug Discovery: Used in high-throughput screening (HTS) assays to evaluate the effects of pharmaceutical compounds on calcium-dependent cellular pathways and GPCR signaling.
• Cell Physiology Studies: Investigating calcium's role as a second messenger in processes like muscle contraction, cell proliferation, apoptosis, and secretion.
• Diagnostic Assay Development: Serving as a key component in the development of in vitro diagnostic kits for assessing cellular health and function.

Basic Information

Item	Detail
Product Name	Fura 2 Pentapotassium Salt
CAS No.	113694-64-7
Molecular Formula	C₂₄H₂₀K₅N₃O₁₅
Molecular Weight	831.99 g/mol
Synonyms	Fura-2, pentapotassium salt; Fura-2, K⁺ salt; Fura 2, pentapotassium; 1-[6-Amino-2-(5-carboxy-2-oxazolyl)-5-benzofuranyloxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid pentapotassium salt; Fura-2 pentapotassium; Calcium indicator Fura-2 K⁺ salt; Bis-(aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (Fura-2) pentapotassium salt
EINECS	Contact for details

Quality Control

Our Fura 2 Pentapotassium Salt is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification, spectrophotometry for absorbance profile confirmation, and functional validation in calcium buffering assays. A detailed Certificate of Analysis (COA) documenting identity, purity, and performance characteristics is provided with every shipment to guarantee it meets the exacting standards required for life science research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, light, or ambient temperature will degrade product performance. Aliquot into single-use vials upon receipt to minimize freeze-thaw cycles and maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Calcium Binding Affinity (Kd)	~135 nM (at 22°C, pH 7.05)
Excitation/Emission (Ca²⁺ free)	~363 nm / ~512 nm
Excitation/Emission (Ca²⁺ bound)	~335 nm / ~505 nm
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.