Description

Indo 1-Am is a high-purity organic chemical compound, also known as Indo-1 AM, primarily utilized as a cell-permeant fluorescent calcium indicator in life science research. Its core value lies in its ability to undergo a significant spectral shift upon binding to calcium ions, enabling precise ratiometric measurement of intracellular calcium concentration. This product is essential for researchers and professionals in biochemistry, cell biology, and neuroscience who require reliable tools for studying calcium signaling, cellular physiology, and related pharmacological mechanisms.

Application

• Intracellular Calcium Imaging: As a ratiometric fluorescent probe for quantifying cytosolic free Ca²⁺ concentration ([Ca²⁺]i) in live cells using fluorescence microscopy or flow cytometry.
• Neuroscience Research: For studying calcium dynamics in neurons, including synaptic transmission, action potentials, and neurotransmitter release.
• Cardiovascular and Muscle Physiology: To investigate calcium signaling in cardiac myocytes, smooth muscle cells, and skeletal muscle fibers.
• Pharmacology and Drug Discovery: Used in high-throughput screening (HTS) assays to evaluate the effects of pharmaceutical compounds on calcium channel activity and intracellular signaling pathways.
• Cell Biology Studies: For monitoring calcium fluxes during processes such as apoptosis, cell cycle progression, fertilization, and cell migration.
• Diagnostic Reagent Development: As a key component in the formulation of diagnostic kits and assays for research and clinical applications.

Basic Information

Product Name	Indo 1-Am
CAS No.	112926-02-0
Molecular Formula	C₄₇H₅₈N₄O₁₈
Molecular Weight	967.0 g/mol
Synonyms	Indo-1 AM; Indo-1 Acetoxymethyl Ester; Indo-1 AM Ester; 1-[2-Amino-5-(6-carboxyindol-2-yl)phenoxy]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic Acid Acetoxymethyl Ester; 1-[2-Amino-5-(2-carboxy-3-indolyl)phenoxy]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic Acid Tetrakis(Acetoxymethyl) Ester; Indo-1, AM; Indo-1, Acetoxymethyl Ester, Cell Permeant; Calcium Indicator Indo-1 AM
EINECS	Contact for details

Quality Control

Our Indo 1-Am is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectral analysis for correct fluorescence properties, and functional validation in cell-based assays. We provide a detailed Certificate of Analysis (COA) with every shipment, documenting key parameters to support your research integrity. Our quality system is designed to meet the rigorous standards required for life science and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, it is recommended to store desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Off-white to yellow solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Fluorescence Properties	Conforms to reference
Functional Activity (in cells)	Pass

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.