Description

5(6)-Carboxy-2' 7'-Dichlorofluorescein is a high-purity, functionalized fluorescein derivative used as a critical intermediate and labeling reagent. This compound matters for its role in synthesizing advanced fluorescent probes and dyes with enhanced photostability and specific reactivity. It is primarily needed by manufacturers and researchers in the pharmaceutical, biotechnology, and diagnostic industries for developing assays, sensors, and bio-conjugates.

Application

• Fluorescent Probe Synthesis: A key precursor for creating sensitive and selective fluorescent labels and tags for biomolecules.
• Bioconjugation & Labeling: Used to label antibodies, proteins, and nucleic acids for fluorescence microscopy, flow cytometry, and immunoassays (e.g., ELISA).
• Diagnostic Reagent Manufacturing: Integral in the production of reagents for clinical diagnostics and research kits.
• Sensor Development: Employed in the design of chemical and biological sensors for detecting pH, metal ions, or enzymatic activity.
• Dye Intermediate: Serves as a building block for synthesizing specialized xanthene dyes with modified spectral properties.
• Life Science Research: Used in cellular imaging and tracking studies due to its derivatization potential for cell-permeable probes.

Basic Information

Item	Detail
Product Name	5(6)-Carboxy-2' 7'-Dichlorofluorescein
CAS No.	111843-78-8
Molecular Formula	C21H10Cl2O7
Molecular Weight	445.21 g/mol
Synonyms	5(6)-Carboxy-2',7'-dichlorofluorescein; 2',7'-Dichloro-5(6)-carboxyfluorescein; 5(6)-Carboxy-2',7'-dichloro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; 5(6)-FAM acid, dichloro; Dichloro-5(6)-carboxyfluorescein; 5(6)-Carboxy-2',7'-dichloro-3',6'-dihydroxyfluoran; DCDHF (commonly used abbreviation)
EINECS	Contact for details

Quality Control

Our 5(6)-Carboxy-2' 7'-Dichlorofluorescein is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive applications. A comprehensive Certificate of Analysis (COA) detailing purity, impurities, and physical characteristics is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption, which can affect stability and performance.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0% (Area %)
Water Content (KF)	≤ 3.0%
Residue on Ignition	≤ 0.5%
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.