Description

Bis(1,3-Dibutylbarbiturate)Trimethine Oxonol CAS NO 110425-49-5 is a specialized oxonol dye belonging to the barbiturate class of compounds. This chemical is valued for its role as a sensitive potentiometric probe and membrane potential indicator in biophysical and biochemical research. It is primarily required by research institutions, diagnostic kit manufacturers, and pharmaceutical R&D departments for applications in cellular physiology, neuroscience, and high-throughput screening assays.

Application

• Membrane Potential Sensing: Used as a fluorescent or absorbance probe for monitoring changes in membrane potential in cells, organelles, and artificial lipid bilayers.
• Biophysical Research: Employed in the study of ion channel activity, mitochondrial function, and cellular energetics.
• High-Throughput Screening (HTS): Acts as a key component in assay development for drug discovery, particularly for targets affecting cellular electrophysiology.
• Diagnostic Assays: Utilized in the formulation of research-use-only (RUO) kits for measuring cellular viability and metabolic activity.
• Neuroscience Studies: Applied in neuronal and synaptic activity research to visualize depolarization and hyperpolarization events.

Basic Information

Product Name	Bis(1,3-Dibutylbarbiturate)Trimethine Oxonol
CAS No.	110425-49-5
Molecular Formula	C27H36N4O6
Molecular Weight	512.60 g/mol
Synonyms	DiBAC4(3); Bis-(1,3-dibutylbarbituric acid) trimethine oxonol; Oxonol V; 1,3-Dibutyl-5-[5-(1,3-dibutyl-2,4,6-trioxo-5-hexahydropyrimidin-5-ylidene)-1,3-pentadienyl]-2,4,6(1H,3H,5H)-pyrimidinetrione; Potential-sensitive oxonol dye; BBOT; Membrane potential indicator dye
EINECS	Contact for details

Quality Control

Our Bis(1,3-Dibutylbarbiturate)Trimethine Oxonol is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectral analysis for identity confirmation. A Certificate of Analysis (COA) detailing purity, absorbance characteristics, and other critical parameters is provided with every shipment to support your quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Dark green to black crystalline powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Absorbance Maximum (λmax)	590 - 610 nm (in methanol)
Extinction Coefficient	Contact for details
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.