Description

n-Fmoc-N'-Trityl-L-Histidine Pentafluorophenyl Ester is a highly specialized, protected amino acid derivative designed for solid-phase peptide synthesis (SPPS). This compound is critical for enabling the efficient and selective incorporation of the challenging histidine residue into peptide chains, preventing unwanted side reactions. It is an essential building block for researchers and manufacturers in pharmaceutical development, particularly for creating therapeutic peptides, peptidomimetics, and biochemical probes.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key activated ester for the controlled, racemization-free introduction of histidine into growing peptide sequences.
• Pharmaceutical R&D: Used in the discovery and development of novel peptide-based drugs, including hormones, antibiotics, and enzyme inhibitors.
• Bioconjugation: Serves as a reagent for labeling proteins or other biomolecules with histidine-containing tags or probes.
• Combinatorial Chemistry: Employed in the generation of diverse peptide libraries for high-throughput screening against biological targets.
• Academic Research: A fundamental tool for studying protein structure-function relationships, enzyme mechanisms, and molecular recognition.
• Custom Peptide Production: Essential for contract research and manufacturing organizations (CROs/CMOs) producing bespoke peptides for clients.

Basic Information

Product Name	n-Fmoc-N'-Trityl-L-Histidine Pentafluorophenyl Ester
CAS No.	109434-24-4
Molecular Formula	C46H36F5N3O5
Molecular Weight	805.80 g/mol
Synonyms	Fmoc-His(Trt)-OPfp; N-α-Fmoc-N-π-trityl-L-histidine pentafluorophenyl ester; Fmoc-L-histidine(N-π-trityl)-OPfp ester; (S)-2-(((9H-Fluoren-9-yl)methoxy)carbonylamino)-3-(1-(tritylamino)-1H-imidazol-4-yl)propanoic acid 2,3,4,5,6-pentafluorophenyl ester; Fmoc-His(Trt)-OPfp-OH; Pentafluorophenyl N-[(9H-fluoren-9-ylmethoxy)carbonyl]-N'-trityl-L-histidinate
EINECS	Contact for details

Quality Control

Our n-Fmoc-N'-Trityl-L-Histidine Pentafluorophenyl Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and chiral integrity, NMR for structural confirmation, and MS for identity verification. Certificates of Analysis (COA) detailing all specifications are provided to ensure compliance with research and cGMP-grade material requirements for preclinical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This material is hygroscopic (moisture-sensitive) and light-sensitive; allow the sealed container to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, keep desiccated at low temperature.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Optical Rotation	[α]²⁰D: Contact for details
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.