Description

2'-Iododihydrorhodamine 123 is a specialized derivative of the classic fluorescent dye Rhodamine 123, distinguished by the introduction of an iodine atom. This modification is critical for its primary role as a highly sensitive and selective fluorescent probe for the detection of reactive oxygen species, particularly peroxynitrite, in biological research. It is an essential tool for researchers and scientists in the fields of cell biology, neuroscience, and pharmacology who require precise measurement of oxidative stress and nitrosative stress in live cells and tissues.

Application

• Detection of Reactive Oxygen/Nitrogen Species (ROS/RNS): Specifically used as a selective fluorescent probe for peroxynitrite (ONOO⁻) in cellular assays.
• Oxidative Stress Research: Monitoring intracellular oxidative stress levels in live-cell imaging and flow cytometry experiments.
• Neurodegenerative Disease Studies: Investigating the role of peroxynitrite in models of diseases like Alzheimer's, Parkinson's, and stroke.
• Cardiovascular Research: Assessing oxidative damage and inflammation in cardiac and endothelial cells.
• Drug Discovery & Toxicology: Screening potential therapeutic compounds for their antioxidant or pro-oxidant effects.
• Mitochondrial Function Analysis: Used in conjunction with other dyes to study mitochondrial health and ROS production.

Basic Information

Product Name	2'-Iododihydrorhodamine 123
CAS No.	109282-63-5
Molecular Formula	C21H19IN2O3
Molecular Weight	498.29 g/mol
Synonyms	2'-I-DHR 123; I-DHR; Iododihydrorhodamine 123; Dihydrorhodamine 123 Iodide Derivative; Peroxynitrite Sensor Dye; Reactive Oxygen Species Probe; 2'-Iodo-2,7-dichlorodihydrofluorescein diacetate analog; Fluorescent Peroxynitrite Indicator
EINECS	Contact for details

Quality Control

Our 2'-Iododihydrorhodamine 123 is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity verification and spectroscopic methods for identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data is provided with every shipment to support your research documentation and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to light, moisture, or elevated temperatures will degrade its performance. For long-term stability, store aliquots in a freezer.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Fluorescence Properties	Ex/Em: ~500/525 nm (upon oxidation)
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.