Description

Dihydrorhodamine 123 is a non-fluorescent, cell-permeable derivative of the classic fluorescent dye Rhodamine 123. This compound is critically important as a highly sensitive and specific probe for the detection of reactive oxygen species (ROS), particularly hydrogen peroxide and peroxynitrite, within biological systems. It is an essential research tool for scientists and quality control professionals in the pharmaceutical, biotechnology, and life science research industries, enabling vital studies in oxidative stress, cell viability, and mitochondrial function.

Application

• ROS Detection and Quantification: Primary use as a fluorogenic substrate for detecting intracellular hydrogen peroxide (H₂O₂) and peroxynitrite (ONOO⁻) in cell-based assays.
• Oxidative Stress Research: Critical for studying oxidative stress mechanisms in models of neurodegeneration, cardiovascular disease, inflammation, and aging.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to evaluate the antioxidant or pro-oxidant activity of novel drug candidates.
• Mitochondrial Function Analysis: Employed in assays investigating mitochondrial membrane potential and the role of mitochondria in ROS production.
• Cell Viability and Apoptosis Assays: Integrated into multiplex assays to correlate ROS generation with cell health and death pathways.
• Diagnostic Kit Component: Serves as a key reagent in the formulation of commercial research ELISA kits and diagnostic kits for oxidative stress markers.

Basic Information

Product Name	Dihydrorhodamine 123
CAS No.	109244-58-8
Molecular Formula	C₂₁H₁₈ClN₃O₃
Molecular Weight	395.84 g/mol
Synonyms	DHR 123; Dihydro Rhodamine 123; Reduced Rhodamine 123; 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester, dihydrochloride; 9-(2-Carboxyphenyl)-6-diethylamino-3H-xanthen-3-iminium chloride, reduced form; Non-fluorescent Rhodamine 123; ROS probe DHR123
EINECS	Contact for details

Quality Control

Our Dihydrorhodamine 123 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccation and storage under inert atmosphere to maintain optimal stability and performance.

Specification

Item	Specification
Appearance	Off-white to pale yellow powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Fluorescence Background	Low, meets functional assay criteria

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.