Description

Fura 2-Am CAS NO 108964-32-5 is a high-purity, cell-permeant acetoxymethyl (AM) ester derivative of the fluorescent calcium indicator Fura-2. This compound is critical for non-invasive, quantitative measurement of intracellular calcium ion (Ca²⁺) concentrations in live cells using rationetric fluorescence microscopy. It is an essential research tool for scientists and professionals in cell biology, neuroscience, pharmacology, and cardiovascular research who require precise and reliable intracellular calcium signaling data.

Application

• Intracellular Calcium Imaging: Primary use as a rationetric fluorescent dye for quantifying cytosolic free Ca²⁺ levels in live cells using microscopy (e.g., fluorescence microscopy, confocal microscopy).
• Neuroscience Research: Studying calcium dynamics in neurons, including synaptic transmission, neurotransmitter release, and neuronal excitability.
• Cardiovascular Research: Monitoring calcium transients and signaling pathways in cardiomyocytes, smooth muscle cells, and endothelial cells.
• Pharmacology & Drug Discovery: High-throughput screening (HTS) and mechanism-of-action studies for compounds that modulate calcium channels, receptors, or intracellular calcium stores.
• Cell Physiology Studies: Investigating calcium's role in processes such as muscle contraction, cell proliferation, apoptosis (programmed cell death), and enzyme activation.
• Diagnostic Assay Development: Used as a key component in developing in vitro diagnostic (IVD) and research-use-only (RUO) assays for cellular function.

Basic Information

Product Name	Fura 2-Am
CAS No.	108964-32-5
Molecular Formula	C₂₉H₃₀N₂O₁₅
Molecular Weight	646.56 g/mol
Synonyms	Fura-2 AM; Fura-2 Acetoxymethyl Ester; Fura-2, acetoxymethyl ester; Fura-2, AM ester; Fura-2 pentaacetoxymethyl ester; 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxyl]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid pentaacetoxymethyl ester; Fura 2 AM; Fura2AM
EINECS	Contact for details

Quality Control

Our Fura 2-Am is manufactured under strict quality control protocols to ensure the high purity and performance required for sensitive biological research. Each batch is characterized to confirm identity, purity, and functional suitability for calcium imaging. A comprehensive Certificate of Analysis (COA) detailing HPLC purity, fluorescence properties, and absence of critical impurities is provided with every shipment to support your research reproducibility and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. The product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95% (typical)
Water Content (KF)	≤ 1.0%
Fluorescence Properties	Conforms to reference (λex ~340/380 nm, λem ~510 nm)
Functional Test (Cell Loading)	Suitable for intracellular calcium imaging

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.