Description

7-Diethylamino-3-(4'-Isothiocyanatophenyl)-4-Methylcoumarin is a highly functionalized coumarin derivative featuring a reactive isothiocyanate group. This compound is specifically engineered for use as a fluorescent labeling reagent, offering strong conjugation capabilities with biomolecules. It is primarily utilized by researchers and manufacturers in the life sciences and diagnostic industries for applications requiring sensitive detection and analysis.

Application

• Fluorescent Labeling of Proteins and Antibodies: The isothiocyanate group reacts with primary amines to form stable thiourea bonds, enabling the covalent attachment of a bright coumarin fluorophore for detection and imaging.
• Bioconjugation for Diagnostic Assays: Used in the development of fluorescent probes and reagents for ELISA, lateral flow assays, and other immunoassay platforms to enhance sensitivity.
• Cellular and Tissue Staining: Serves as a key component in preparing fluorescent tags for microscopy, flow cytometry, and histochemistry to visualize specific cellular targets.
• Biomolecule Tracking and Detection: Applied in biochemical research to label peptides, oligonucleotides, or other biomolecules to study their interaction, localization, and kinetics.
• Material Science and Sensor Development: Incorporated into polymers or surfaces to create fluorescent sensors for detecting specific analytes or environmental changes.

Basic Information

Product Name	7-Diethylamino-3-(4'-Isothiocyanatophenyl)-4-Methylcoumarin
CAS No.	107743-39-5
Molecular Formula	C21H20N2O2S
Molecular Weight	364.46 g/mol
Synonyms	7-(Diethylamino)-3-(4-isothiocyanatophenyl)-4-methyl-2H-chromen-2-one; 4-Methyl-7-diethylamino-3-(4'-isothiocyanatophenyl)coumarin; Coumarin 6 ITC; C6-ITC; 7-Diethylaminocoumarin-3-isothiocyanate; 3-(4-Isothiocyanatophenyl)-7-(diethylamino)-4-methylcoumarin; Fluorescent Isothiocyanate Dye
EINECS	Contact for details

Quality Control

Our production of 7-Diethylamino-3-(4'-Isothiocyanatophenyl)-4-Methylcoumarin adheres to stringent quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic methods for structural confirmation. A Certificate of Analysis (COA) detailing identity, purity, and relevant specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. Allow the sealed container to reach room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 1.0%
Fluorescence Properties	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.