Description

Neurodye Rh-421 is a high-purity, fluorescent styryl dye widely used as a sensitive molecular probe for monitoring membrane potential changes in biological systems. Its core value lies in its fast response time and high signal-to-noise ratio, enabling precise, real-time electrophysiological measurements. This compound is essential for researchers and manufacturers in the fields of neuroscience, cardiology, and cellular biophysics, as well as for developing diagnostic kits and high-throughput screening assays.

Application

• Neuroscience Research: Primary application for optical monitoring of neuronal action potentials and synaptic activity.
• Cardiac Electrophysiology: Used in studies of heart cell (cardiomyocyte) membrane potential and arrhythmia mechanisms.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify compounds that modulate ion channel function.
• Diagnostic Assay Development: A key component in fluorescence-based kits for assessing cell viability and ion channel activity.
• Cellular Biophysics: Fundamental research tool for studying the kinetics and thermodynamics of voltage-gated ion channels.
• Microbial Physiology: Potential use in probing membrane potential in bacteria and yeast for antimicrobial research.

Basic Information

Product Name	Neurodye Rh-421
CAS No.	107610-19-5
Molecular Formula	C29H31N2O3S+
Molecular Weight	487.63 g/mol
Synonyms	RH 421; RH421; N-(4-Sulfobutyl)-4-(4-(p-Dipentylaminophenyl)butadienyl)pyridinium, Inner Salt; 4-[4-[4-(Dipentylamino)phenyl]-1,3-butadienyl]-1-(4-sulfobutyl)pyridinium, Hydroxide, Inner Salt; Potential-Sensitive Probe RH 421; Voltage-Sensitive Dye RH 421; Styrylpyridinium Dye RH 421
EINECS	Contact for details

Quality Control

Every batch of Neurodye Rh-421 is manufactured and analyzed under strict quality management protocols to ensure exceptional purity and batch-to-batch consistency, critical for sensitive research applications. Our products undergo rigorous quality testing, including HPLC for purity and spectroscopic methods for identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence profiles, and other critical parameters is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. For long-term storage, consider keeping the product desiccated at -20°C. Allow the product to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Dark green to black crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Absorbance (λmax in Methanol)	~ 421 nm
Fluorescence (λmax in Methanol)	~ 500-550 nm (Excitation ~421 nm)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.