Description

Thiazole Orange is a high-purity, asymmetric cyanine dye widely recognized for its exceptional fluorescence properties. This compound is critical for applications requiring sensitive and selective nucleic acid detection due to its significant fluorescence enhancement upon binding. It is an essential reagent for research and diagnostic laboratories in the biotechnology, pharmaceutical, and life sciences sectors, where reliable and high-quality fluorescent markers are paramount.

Application

• Nucleic Acid Staining: Primary use as a fluorescent stain for DNA and RNA in gel electrophoresis (e.g., agarose gels), offering high sensitivity and low background.
• Flow Cytometry: Used as a cell-permeant dye for the quantification of cellular DNA content and cell cycle analysis.
• Fluorescence Microscopy: Employed for the visualization of nucleic acids within fixed cells and tissue sections.
• Real-Time PCR (qPCR): Functions as an intercalating dye for monitoring DNA amplification in real-time, though often in specialized formulations.
• Biophysical Studies: Utilized in research investigating DNA-drug interactions, DNA conformation, and the dynamics of nucleic acid structures.
• Diagnostic Kit Manufacturing: A key component in the production of commercial kits for molecular biology and clinical diagnostics.

Basic Information

Product Name	Thiazole Orange
CAS No.	107091-89-4
Molecular Formula	C21H19N3OS
Molecular Weight	361.46 g/mol
Synonyms	TO; TO-PRO-1; Thiazole orange homodimer; 1,1'-(4,4,7,7-Tetramethyl-4,7-dihydro-5H-4,7-methano-2-benzothiazol-2-ylidene)bis-methine]bis[3-methyl-1,3-benzothiazol-3-ium]; 3-Methyl-2-((E)-3-(1,3,3-trimethyl-5-sulfoindolin-2-ylidene)prop-1-enyl)benzothiazol-3-ium; Thiazole Orange Dye; Benzothiazolium dye
EINECS	Contact for details

Quality Control

Our Thiazole Orange is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including HPLC for purity, spectrophotometry for absorbance/fluorescence verification, and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container should be kept in a desiccated environment to prevent moisture absorption, which can affect performance and stability.

Specification

Item	Specification
Appearance	Orange to dark red powder
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorbance (λmax)	509 ± 5 nm (in methanol)
Fluorescence (λmax)	533 ± 5 nm (when bound to DNA)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.