Description

Fura-2-Am is a high-purity, cell-permeant fluorescent calcium indicator widely used in biological research. Its primary value lies in its ability to selectively chelate intracellular calcium ions, producing a measurable shift in fluorescence emission, which is critical for real-time monitoring of calcium dynamics in living cells. This compound is essential for researchers and professionals in the fields of cell biology, neuroscience, and pharmacology, where precise measurement of intracellular calcium signaling is required.

Application

• Intracellular Calcium Imaging: Primary use for visualizing and quantifying real-time changes in cytosolic free Ca²⁺ concentrations in live cells.
• Neuroscience Research: Studying calcium flux in neurons, synaptic transmission, and neuronal activity.
• Cardiovascular Research: Monitoring calcium transients in cardiomyocytes and vascular smooth muscle cells.
• Drug Discovery & Screening: Used in high-throughput assays to evaluate the effects of pharmacological agents on cellular calcium pathways.
• Cell Signaling Studies: Investigating GPCR (G-protein coupled receptor) activation, ion channel function, and second messenger systems.
• Fluorescence Microscopy & Flow Cytometry: Serving as a key reagent for quantitative fluorescence-based analytical techniques.

Basic Information

Product Name	Fura-2-Am
CAS No.	105344-37-4
Molecular Formula	C₂₉H₂₈N₄O₁₅
Molecular Weight	672.55 g/mol
Synonyms	Fura-2 AM; Fura-2 Acetoxymethyl Ester; Fura-2, AM ester; Fura-2 acetoxymethyl ester; 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxyl]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester; AM ester of Fura-2; Fura-2 pentaacetoxymethyl ester; Fura 2 AM
EINECS	Contact for details

Quality Control

Our Fura-2-Am is manufactured under strict quality protocols to ensure batch-to-batch consistency and performance reliability for sensitive research applications. Each lot is subjected to comprehensive analytical testing, including HPLC for purity, spectral analysis for correct excitation/emission profiles, and functional validation in cell-based assays. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming compliance with our stringent research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Fluorescence Properties	Ex/Em ~340-380/510 nm (Ca²⁺ bound/free shift confirmed)
Functional Activity (Cell Assay)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.