Description

Dihydroethidium is a cell-permeable, blue-fluorescent probe that is specifically oxidized by superoxide to form a red-fluorescent product, ethidium. This compound is a critical reagent for the direct detection and quantification of intracellular superoxide radical (O₂•⁻) production in live cells. It is an essential tool for researchers in life sciences, pharmacology, and toxicology studying oxidative stress, mitochondrial function, and inflammatory responses. Dihydroethidium CAS NO 104821-25-2 offers high sensitivity and specificity for superoxide detection in various cellular and tissue models.

Application

• Detection of Superoxide Anion in Live Cells: Primary application for real-time monitoring of O₂•⁻ generation using fluorescence microscopy or flow cytometry.
• Oxidative Stress Research: Used to assess oxidative damage and antioxidant efficacy in models of neurodegeneration, cardiovascular disease, and aging.
• Mitochondrial Function Assays: Employed to measure superoxide production as a key indicator of mitochondrial health and dysfunction.
• Pharmacology and Drug Screening: Utilized to evaluate the pro-oxidant or antioxidant effects of novel drug candidates and natural compounds.
• Inflammation and Immunology Studies: Applied to detect superoxide burst in immune cells like neutrophils and macrophages during phagocytosis.
• Toxicology Assessments: Serves as a biomarker for cellular oxidative injury induced by environmental toxins or chemicals.

Basic Information

Product Name	Dihydroethidium
CAS No.	104821-25-2
Molecular Formula	C₂₁H₂₁N₃
Molecular Weight	315.41 g/mol
Synonyms	Dihydroethidium; Hydroethidine; DHE; 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine; Reduced Ethidium; Superoxide Indicator; Oxidative Stress Probe; Ethidium homodimer analog
EINECS	Contact for details

Quality Control

Our Dihydroethidium is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is characterized by multiple analytical techniques, including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in a standard assay is provided with every shipment. We adhere to relevant quality management standards to guarantee product integrity and reliability for your critical research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store the powder in a freezer. Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Red to brown powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Performance (Fluorescence Assay)	Exhibits characteristic fluorescence shift upon reaction with superoxide

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.