Description

4-Azido-n-Methylspiperone is a specialized azido-functionalized derivative of the dopamine receptor antagonist spiperone. This compound is primarily valued as a high-affinity, photoactivatable ligand for biochemical research, enabling the covalent cross-linking and study of dopamine D2 and serotonin 5-HT2A receptors. It is an essential tool for researchers and pharmaceutical developers in the fields of neuropharmacology, molecular biology, and medicinal chemistry, facilitating the elucidation of receptor structure and function.

Application

• Photoaffinity Labeling: Acts as a critical probe for covalently tagging and identifying dopamine D2 and serotonin 5-HT2A receptor binding sites upon UV irradiation.
• Neuropharmacology Research: Used in fundamental studies to investigate receptor-ligand interactions, signal transduction pathways, and receptor localization.
• Medicinal Chemistry & Drug Discovery: Serves as a key intermediate or tool compound in the design and development of novel CNS-active pharmaceuticals.
• Biochemical Assay Development: Employed in the creation of specialized assays for high-throughput screening (HTS) and binding affinity measurements.
• Molecular Biology Studies: Facilitates the study of G-protein coupled receptor (GPCR) dynamics, conformation, and oligomerization.
• Radioligand Precursor: Can serve as a precursor for the synthesis of tritiated or iodinated radioligands for autoradiography and binding studies.

Basic Information

Product Name	4-Azido-n-Methylspiperone
CAS No.	104820-55-5
Molecular Formula	C₂₃H₂₆FN₅O₂
Molecular Weight	431.49 g/mol
Synonyms	4-Azido-N-methylspiperone; 8-[4-(4-Azidophenyl)-4-oxobutyl]-1-phenyl-1,3,8-triazaspiro[4.5]decan-4-one; N-Methyl-4-azidospiperone; 4-Azidospiperone N-methyl derivative; Azido-methylspiperone; Photoaffinity label for D2 receptor; D2/5-HT2A photoaffinity ligand
EINECS	Contact for details

Quality Control

Every batch of 4-Azido-n-Methylspiperone is manufactured and analyzed under strict quality management protocols to ensure the highest standards of purity and consistency required for research applications. Our products undergo rigorous quality testing, including HPLC, NMR, and MS analysis, to ensure compliance with research-grade specifications. Certificates of Analysis (COA) detailing purity, identity, and other critical parameters are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. The compound is light-sensitive and should be handled under subdued lighting conditions to prevent degradation. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.