Description

Sulfosuccinimidyl 3-((2-(4-Azidosalicylamido)Ethyl)Dithio)Propionate is a heterobifunctional crosslinking reagent designed for advanced bioconjugation and protein labeling. Its core value lies in its dual functionality, featuring an amine-reactive NHS ester and a photoactivatable aryl azide group, connected by a cleavable disulfide bridge. This makes it an essential tool for researchers and manufacturers in the life sciences, particularly for applications requiring controlled crosslinking, affinity labeling, and the creation of reversible conjugates in proteomics and antibody-drug conjugate (ADC) development.

Application

• Bioconjugation and Protein Labeling: Covalently attaching probes, tags, or drugs to proteins, antibodies, and peptides via amine groups.
• Photoaffinity Labeling: Studying protein-protein interactions and ligand-receptor binding sites using the UV-activatable aryl azide group.
• Controlled Crosslinking: Creating reversible protein complexes or immobilized surfaces where the disulfide bond can be cleaved under reducing conditions for analysis.
• Antibody-Drug Conjugate (ADC) Research: Serving as a linker candidate in the development of next-generation ADCs with cleavable, traceless release mechanisms.
• Surface Modification: Functionalizing chips, beads, or other solid supports for biosensor development and microarray technology.
• Proteomics and Biomarker Discovery: Crosslinking interacting proteins in complex biological samples for subsequent isolation and identification.

Basic Information

Product Name	Sulfosuccinimidyl 3-((2-(4-Azidosalicylamido)Ethyl)Dithio)Propionate
CAS No.	103963-69-5
Molecular Formula	C20H21N5O8S3
Molecular Weight	563.60 g/mol
Synonyms	Sulfo-SDA; SASD; Sulfosuccinimidyl 2-((4-azidosalicylamido)ethyl)dithio)propionate; N-Sulfosuccinimidyl 3-((2-(4-azidosalicylamido)ethyl)dithio)propionate; 3-((2-(4-Azidosalicylamido)ethyl)dithio)propionic acid sulfo-NHS ester; SASD Crosslinker; Heterobifunctional Crosslinker SASD; Photoactivatable NHS Ester Crosslinker
EINECS	Contact for details

Quality Control

Our Sulfosuccinimidyl 3-((2-(4-Azidosalicylamido)Ethyl)Dithio)Propionate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment, NMR and MS for structural confirmation, and functional validation in standard conjugation assays. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance characteristics is provided with every shipment to ensure consistency and reliability for your critical research and development.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity	Conforms (by conjugation assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.