Description

Cholesteryl 5-Carboxypentyl Ether n-Hydroxysuccinimide Ester CAS NO 103003-22-1 is a high-purity, functionalized cholesterol derivative designed for advanced bioconjugation and labeling applications. This compound is a critical reagent for researchers and manufacturers requiring stable, covalent attachment of cholesterol moieties to proteins, peptides, or other biomolecules. It is essential for professionals in pharmaceutical R&D, advanced materials science, and diagnostic assay development who need to modify lipid profiles or create targeted delivery systems with precision and reliability.

Application

• Bioconjugation Reagent: For the covalent attachment of cholesterol to antibodies, proteins, or peptides to modulate hydrophobicity, membrane targeting, or circulation half-life.
• Lipid Nanoparticle (LNP) Formulation: Used in the research and development of novel LNP systems for mRNA, siRNA, or other nucleic acid delivery platforms.
• Fluorescent Probe Synthesis: Acts as a key intermediate for synthesizing cholesterol-based fluorescent dyes for cellular imaging and membrane dynamics studies.
• Biomaterial Functionalization: Enables the surface modification of polymers or nanoparticles with cholesterol to enhance biocompatibility or specific cellular interactions.
• Diagnostic Assay Development: Utilized in creating components for immunoassays or biosensors that require cholesterol tagging for signal amplification or capture.
• Pharmaceutical Research: Serves as a building block in prodrug design or the synthesis of novel therapeutic agents targeting lipid metabolism or membrane receptors.

Basic Information

Product Name	Cholesteryl 5-Carboxypentyl Ether n-Hydroxysuccinimide Ester
CAS No.	103003-22-1
Molecular Formula	C37H59NO5
Molecular Weight	597.87 g/mol
Synonyms	Cholesteryl N-succinimidyl 5-carboxypentyl ether; Cholesterol NHS Ester (C6 linker); 5-[(Cholest-5-en-3β-yloxy)carbonyl]pentanoic Acid N-Hydroxysuccinimide Ester; N-Hydroxysuccinimide Ester of Cholesteryl 5-Carboxypentyl Ether; 3β-[[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]oxy]cholest-5-ene; Cholesteryl Hemisuccinate NHS Ester analog (C6); Cholesterol-PEG5-NHS Ester (approximate); Chol-C6-NHS
EINECS	Contact for details

Quality Control

Every batch of Cholesteryl 5-Carboxypentyl Ether n-Hydroxysuccinimide Ester is manufactured and tested under strict quality management systems to ensure the highest standards of purity and consistency for research and production. Our products undergo rigorous analytical characterization, including HPLC, NMR, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available for every lot upon request, supporting compliance in cGMP and ISO environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and hydrolysis. For long-term stability, store under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.