Description

Ethyl 4-Azidophenyl-1,4-Dithiobutyrimidate is a specialized bifunctional chemical reagent featuring both an azido group and a thioester moiety, making it a valuable intermediate for click chemistry and bioconjugation applications. Its primary value lies in enabling efficient, site-specific cross-linking and labeling of biomolecules under mild conditions, which is critical for advanced research and development. This compound is essential for scientists and manufacturers in the pharmaceutical, biotechnology, and life science sectors engaged in proteomics, antibody-drug conjugate (ADC) development, and materials surface functionalization.

Application

• Bioconjugation Reagent: Used for site-specific labeling of proteins, antibodies, and peptides via copper-free or strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry.
• Antibody-Drug Conjugate (ADC) Linker Precursor: Serves as a key intermediate in the synthesis of cleavable or non-cleavable linkers for targeted cancer therapies.
• Polymer and Materials Science: Functionalizes surfaces and polymers to introduce bioactive groups for creating smart materials, biosensors, and diagnostic devices.
• Chemical Biology Probes: Enables the synthesis of activity-based probes for studying enzyme function and protein-protein interactions in complex biological systems.
• Peptide and Protein Modification: Facilitates the introduction of stable thioether linkages or fluorescent tags for tracking and analysis.
• Diagnostic Assay Development: Used in the fabrication of immunoassays and other diagnostic platforms requiring stable, covalent immobilization of capture molecules.

Basic Information

Product Name	Ethyl 4-Azidophenyl-1,4-Dithiobutyrimidate
CAS No.	102568-44-5
Molecular Formula	C13H16N4OS2
Molecular Weight	308.42 g/mol
Synonyms	Ethyl 4-Azidobenzimidate, 1,4-Dithiobutyrimidate; 4-Azidophenyl-1,4-dithiobutyrimidic Acid Ethyl Ester; S-[2-[(4-Azidophenyl)imino]ethyl] ethanethioate; Azido-PEG4-Thioacetate Ester (related); Bioconjugation Crosslinker; Click Chemistry Reagent; ADC Linker Intermediate
EINECS	Contact for details

Quality Control

Our Ethyl 4-Azidophenyl-1,4-Dithiobutyrimidate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and LC-MS for identity verification. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all specifications and test results to ensure compliance with your research and cGMP-grade application requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) for long-term stability. The compound is light-sensitive (store away from light) and may be moisture-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.