Description

5-(4,6-Dichlorotriazinyl)Aminofluorescein is a highly reactive, amine-reactive fluorescent labeling reagent derived from fluorescein. This compound matters for researchers and manufacturers who require a stable, covalent method to label proteins, antibodies, and other biomolecules with a bright, pH-insensitive fluorescent tag for detection and analysis. It is essential for professionals in bioconjugation, diagnostic assay development, and fluorescence microscopy who need reliable, high-purity reagents for sensitive applications.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to primary amines on proteins, peptides, antibodies, and oligonucleotides for detection and quantification.
• Diagnostic Assay Development: Used as a critical component in the manufacture of fluorescent probes and reagents for ELISA, lateral flow assays, and other immunoassays.
• Cell and Tissue Staining: Preparation of fluorescent conjugates for fluorescence-activated cell sorting (FACS) and confocal microscopy imaging.
• Bioconjugation Research: A key tool in chemical biology for studying protein-protein interactions, localization, and dynamics.
• Material Science: Functionalization of surfaces and polymers with fluorescent markers for sensor development and biointerface studies.

Basic Information

Product Name	5-(4,6-Dichlorotriazinyl)Aminofluorescein
CAS No.	102417-95-8
Molecular Formula	C23H12Cl2N4O5
Molecular Weight	495.27 g/mol
Synonyms	5-DTAF; 5-(4,6-Dichloro-1,3,5-triazin-2-yl)aminofluorescein; Aminofluorescein DCT; DCT-Aminofluorescein; 5-[(4,6-Dichloro-1,3,5-triazin-2-yl)amino]fluorescein; Fluorescein-5-isothiocyanate analogue (reactive dichlorotriazine); DTAF; 5-(4,6-Dichloro-s-triazin-2-yl)aminofluorescein
EINECS	Contact for details

Quality Control

Our 5-(4,6-Dichlorotriazinyl)Aminofluorescein is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for labeling efficiency verification, and identity confirmation. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is light-sensitive; prolonged exposure to light should be avoided to maintain reactivity and fluorescence properties. Keep the container sealed in a desiccator under inert atmosphere for long-term storage to prevent moisture absorption and degradation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum	490 - 495 nm
Emission Maximum	515 - 520 nm
Extinction Coefficient	~80,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.