Description

ε-n-Maleimidocaproic Acid-(2-Nitro-4-Sulfo)-Phenyl Ester Sodium Salt is a heterobifunctional crosslinking reagent featuring a maleimide group and an amine-reactive NHS ester, separated by a long, hydrophilic spacer arm. This compound is engineered to enable the stable, site-specific conjugation of biomolecules, such as antibodies and proteins, to other entities like peptides, drugs, or surfaces. It is an essential tool for researchers and manufacturers in bioconjugation, diagnostic assay development, and targeted drug delivery systems, where controlled and efficient coupling is critical.

Application

• Antibody-Drug Conjugate (ADC) Linker: For the covalent attachment of cytotoxic payloads to monoclonal antibodies via thiol-maleimide chemistry.
• Protein and Peptide Labeling: Conjugation of fluorescent dyes, enzymes, or biotin to proteins for detection and imaging applications.
• Diagnostic Assay Development: Immobilization of capture antibodies or antigens onto activated surfaces for ELISA and lateral flow assays.
• Surface Functionalization: Modification of gold nanoparticles, magnetic beads, or sensor chips with biomolecules for biosensing and separation technologies.
• Polymer-Protein Conjugates: Crafting PEGylated proteins or other polymer-biomolecule hybrids to improve pharmacokinetic properties.
• Stable Complex Formation: Creating irreversible crosslinks between interacting proteins for structural studies or affinity purification.

Basic Information

Product Name	ε-n-Maleimidocaproic Acid-(2-Nitro-4-Sulfo)-Phenyl Ester Sodium Salt
CAS No.	101554-76-1
Molecular Formula	C21H22N3NaO11S
Molecular Weight	547.47 g/mol
Synonyms	NHS-PEG6-Maleimide, Sodium Salt; Maleimide-PEG6-NHS Ester; SM(PEG)6; 6-((6-((2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)oxy)-6-oxohexyl)amino)-6-oxohexyl (2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl) (2-nitro-4-sulfophenyl) carbonate sodium salt; m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester (sulfo-MBS) analog with extended spacer; Sulfo-EMCS; Heterobifunctional Crosslinker, NHS-PEG-Maleimide
EINECS	Contact for details

Quality Control

Our ε-n-Maleimidocaproic Acid-(2-Nitro-4-Sulfo)-Phenyl Ester Sodium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for sensitive bioconjugation work. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.