Description

5'-o-tert-Butyldiphenylsilyl-Thymidine is a key nucleoside derivative, specifically a thymidine analog protected at the 5'-hydroxyl position with a tert-butyldiphenylsilyl (TBDPS) group. This compound is critical for the synthesis of oligonucleotides and modified nucleic acids, where it serves as a vital building block to ensure regioselective control and prevent unwanted side reactions. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for developing novel therapeutics, probes, and research tools.

Application

• Oligonucleotide Synthesis: A fundamental phosphoramidite precursor in solid-phase synthesis for creating DNA and RNA strands.
• Antiviral & Anticancer Prodrug Development: Used in the research and production of nucleoside-based prodrugs targeting viral infections and cancers.
• Diagnostic Probe Manufacturing: Essential for constructing labeled nucleotides used in fluorescence in situ hybridization (FISH) and other molecular diagnostic assays.
• Medicinal Chemistry Research: Serves as a protected intermediate in the structure-activity relationship (SAR) studies of novel nucleoside analogs.
• Bioconjugation & Labeling: The protected hydroxyl allows for selective functionalization, enabling the attachment of reporter molecules or linkers.

Basic Information

Product Name	5'-o-tert-Butyldiphenylsilyl-Thymidine
CAS No.	101527-40-6
Molecular Formula	C26H32N2O6Si
Molecular Weight	496.64 g/mol
Synonyms	5'-O-(tert-Butyldiphenylsilyl)thymidine; 5'-O-TBDPS-Thymidine; 1-[(2R,4S,5R)-4-Hydroxy-5-[[tert-butyl(diphenyl)silyl]oxymethyl]oxolan-2-yl]-5-methylpyrimidine-2,4-dione; Thymidine 5'-O-tert-butyldiphenylsilyl ether; TBDPS-Thymidine; 5'-O-(tert-Butyldiphenylsilyl)-2'-deoxythymidine
EINECS	Contact for details

Quality Control

Our 5'-o-tert-Butyldiphenylsilyl-Thymidine is produced under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for demanding research and cGMP manufacturing applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards, and specifications can be tailored to meet specific project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.0%
Water Content (KF)	≤0.5%
Related Substances (HPLC)	Total impurities ≤2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.