Description

4',5'-Dimethyl-5-Carboxyfluorescein is a chemically modified derivative of fluorescein, a cornerstone fluorophore in life science research. This compound matters for its enhanced photostability and specific spectral properties, making it a superior choice for creating highly sensitive and stable fluorescent probes and labels. It is primarily needed by researchers and manufacturers in the fields of molecular diagnostics, biochemistry, and analytical chemistry for applications such as nucleic acid labeling and fluorescence-based assays.

Application

• Fluorescent Labeling Reagent: Used as an active ester (e.g., NHS ester) for covalently labeling biomolecules like proteins, peptides, and oligonucleotides.
• Molecular Probes: A key precursor for synthesizing fluorescent probes and sensors for detecting ions, enzymes, or other analytes.
• Nucleic Acid Analysis: Employed in the synthesis of labeled nucleotides for DNA sequencing, PCR, and fluorescence in situ hybridization (FISH).
• Cell Biology: Used to prepare fluorescent tracers for studying cell permeability, viability, and intracellular pH.
• Immunoassays: Serves as a fluorescent tag for antibodies in ELISA and other immunoassay techniques to enhance detection sensitivity.
• Capillary Electrophoresis: Acts as a high-sensitivity fluorescent marker for the separation and analysis of biomolecules.
• Material Science: Incorporated into polymers or nanoparticles to create functional fluorescent materials.

Basic Information

Product Name	4',5'-Dimethyl-5-Carboxyfluorescein
CAS No.	100111-02-2
Molecular Formula	C24H18O7
Molecular Weight	418.40 g/mol
Synonyms	5-Carboxy-4',5'-dimethylfluorescein; 5(6)-Carboxy-2',7'-dimethylfluorescein; 2',7'-Dimethyl-5(6)-carboxyfluorescein; 4',5'-Dimethylfluorescein-5(6)-carboxylic acid; DMFCA; 5(6)-DMFCA; 4',5'-Dimethyl-5(6)-carboxyfluorescein; 5-Carboxy-2',7'-dimethylfluorescein
EINECS	Contact for details

Quality Control

Our 4',5'-Dimethyl-5-Carboxyfluorescein is produced under strict quality management systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and structural confirmation via spectroscopic methods. A detailed Certificate of Analysis (COA) providing lot-specific data is available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). Keep the container sealed to protect this moisture-sensitive material from humidity.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Fluorescence Properties	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.