Description

Cresyl Violet is a synthetic basic dye belonging to the phenoxazine class, widely recognized for its metachromatic staining properties. This compound is essential for histological and cytological applications, providing critical contrast and definition to biological specimens. It is a fundamental reagent for researchers and diagnostic laboratories in the life sciences, neuroscience, and pathology sectors, where precise cellular visualization is paramount.

Application

• Histological Staining: A primary stain for Nissl substance in neurons, enabling the study of neuronal cell bodies and cytoarchitecture in brain tissue sections.
• Cytological Analysis: Used in staining procedures for blood cells and bone marrow smears in hematology and clinical diagnostics.
• Microbiology: Employed as a counterstain in specific bacterial staining protocols to enhance microscopic visibility.
• Metachromatic Staining: Useful for identifying and characterizing acidic tissue components like mucins, cartilage, and mast cell granules due to its color-shift properties.
• Neuroscience Research: Critical for brain mapping, lesion studies, and quantifying neuronal populations in experimental models.
• Educational Kits: A standard component in prepared staining kits for university and medical school teaching laboratories.

Basic Information

Product Name	Cresyl Violet
CAS No.	18472-89-4
Molecular Formula	C19H18ClN3O
Molecular Weight	339.82 g/mol
Synonyms	Cresyl Violet Acetate; Cresylecht Violet; C.I. 51010; Basic Violet 10; Cresyl Violet Perchlorate (common salt form); 9-Amino-5-imino-5H-benzo[a]phenoxazine; 9-Amino-5-iminodibenzo[a,j]phenoxazine
EINECS	242-355-8

Quality Control

Our Cresyl Violet is manufactured under a strict quality management system. Every batch undergoes comprehensive analytical testing to ensure identity, purity, and performance consistency suitable for diagnostic and research applications. A detailed Certificate of Analysis (COA) documenting purity, absorbance profile, and solubility is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	Dark green to violet powder or crystals
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Absorbance (λmax)	Characteristic peak in ethanol
Solubility	Soluble in water and ethanol
Loss on Drying	≤ 2.0 %
Residue on Ignition	≤ 1.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.