Description

Phloxinbformicroscopy CAS NO 18472-84-2 is a specialized biological stain, also known as Phloxine B, primarily used as a fluorescent dye in microscopy. This compound is valued for its ability to produce a bright red fluorescence under specific conditions, providing high contrast and clarity in cellular and histological imaging. It is an essential reagent for researchers and technicians in life sciences, pathology, and biotechnology who require reliable and consistent staining for diagnostic and analytical procedures.

Application

• Histological Staining: Used as a counterstain in combination with other dyes (e.g., methyl green) for visualizing cell nuclei and cytoplasmic structures in tissue sections.
• Fluorescence Microscopy: Employed as a fluorescent probe for labeling and imaging biological samples, including bacteria, yeast, and mammalian cells.
• Viability Assays: Applied in protocols to distinguish between live and dead cells based on membrane permeability.
• Botanical Studies: Utilized for staining plant tissues and pollen grains to enhance microscopic examination.
• Textile Dyeing: Serves as a colorant for silk, wool, and other protein-based fibers.
• Cosmetic and Food Coloring: Approved in some regions (where regulated) as a red color additive in cosmetics and certain food products.
• Ink Formulation: Used in the manufacture of inks for specialized printing applications.
• Research & Development: A key tool in biochemical and pharmaceutical research for developing new staining protocols and diagnostic kits.

Basic Information

Item	Details
Product Name	Phloxinbformicroscopy
CAS No.	18472-84-2
Molecular Formula	C20H2Br4Cl4Na2O5
Molecular Weight	829.64 g/mol
Synonyms	Phloxine B; Acid Red 92; C.I. 45410; D&C Red No. 28; Eosin 10B; Eosin Y; Eosine Y; Eosine Yellowish; 2',4',5',7'-Tetrabromo-4,5,6,7-tetrachloro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one disodium salt; Eosine B; Bromoeosine
EINECS	242-355-6

Quality Control

Our Phloxinbformicroscopy is produced under strict quality management systems to ensure batch-to-batch consistency suitable for research and industrial applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectrophotometric analysis for dye content. While not necessarily manufactured to a specific pharmacopeial monograph, our quality standards align with the rigor expected for biological stains. A Certificate of Analysis (COA) detailing identity, purity, and performance characteristics is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive; prolonged exposure to light may cause degradation and loss of staining performance. Keep the container tightly sealed when not in use to protect from moisture.

Specification

Item	Specification
Appearance	Red to dark red powder
Identification (IR)	Conforms to structure
Assay (Dye Content)	≥ 85%
Loss on Drying	≤ 5.0%
Absorbance (λ max)	540 - 550 nm
Heavy Metals (as Pb)	≤ 20 ppm
Solubility (in Water)	Clear, red solution
Performance (Staining Test)	Passes visual microscopy test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.