Description

Acridineorangeformicroscopicalstaining CAS NO 17251-70-6 is a vital fluorescent cationic dye and nucleic acid-selective stain used extensively in biological research and diagnostics. Its core value lies in its metachromatic properties, allowing for the differential staining of DNA and RNA, which is crucial for cell cycle analysis and apoptosis detection. This product is essential for researchers, clinical laboratories, and pharmaceutical developers engaged in fluorescence microscopy, flow cytometry, and vital staining procedures.

Application

• Fluorescence Microscopy: Primary application for visualizing and differentiating cellular nucleic acids (DNA vs. RNA) in fixed and live cells.
• Flow Cytometry: Used for cell cycle analysis and the quantification of cellular DNA/RNA content.
• Apoptosis Detection: Aids in identifying programmed cell death due to its differential uptake by live, apoptotic, and necrotic cells.
• Microbiological Staining: Employed as a fluorescent stain for bacteria and other microorganisms.
• Vital Staining: Suitable for staining living cells without immediate toxicity, allowing for real-time observation.
• Histology & Cytology: Used in specialized staining protocols for tissue sections and smears to highlight nucleic acid structures.
• Research & Development: A key tool in molecular biology, cancer research, and toxicology studies for assessing cell viability and proliferation.

Basic Information

Product Name	Acridineorangeformicroscopicalstaining
CAS No.	17251-70-6
Molecular Formula	C₁₇H₁₉N₃
Molecular Weight	265.36 g/mol
Synonyms	Acridine Orange; Acridine Orange base; 3,6-Bis(dimethylamino)acridine; Basic Orange 14; C.I. 46005; Acridine Orange Stain; Acridine Orange Hydrochloride (salt form); AO Stain
EINECS	Contact for details

Quality Control

Our Acridine Orange is produced and tested to meet the stringent requirements of research and diagnostic applications. Each batch undergoes rigorous quality testing, including purity assay and spectral verification, to ensure consistent performance and batch-to-batch reproducibility. Certificates of Analysis (COA) detailing specific results for identity, purity, and performance in staining are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at a controlled room temperature (15-25°C) in a dry environment. The product is light-sensitive; prolonged exposure to light should be avoided to prevent degradation.

Specification

Item	Specification
Appearance	Orange to brownish-orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Fluorescence Properties	Conforms to standard (λex/λem)
Solubility	Soluble in water and ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.